The effects of cyclopiazonic acid (CPA), a known Ca2'-pump inhibitor at internal stores, were investigated on electrical responses of the membrane of smooth muscle cells in small segments (O.3-O.5 mm long) of circular smooth muscle isolated from the guineapig gastric antrum. In most preparations, the membrane was spontaneously active with the generation of unitary potentials and regenerative sl...

The aim of this study was to identify the effects highly dispersed silica nanoparticles (HDSns) on cryoresistance Ovis Aries cumulus oocytes in conditions intra-(IOV) or extra-(EOV) ovarian vitrification. Materials and methods. Cumulus-oocyte complexes (COC) intended for EOV were treated with solutions cryoprotectants (CPA) prepared TC-199 10% FBS (fetal bovine serum): CPA-1-0.7 M dimethyl sulf...

We examined the antitumor efficacy of the capecitabine (CAPE) plus cyclophosphamide (CPA) combination as a 2-line therapy after paclitaxel (PTX) plus bevacizumab (BEV) treatment in a xenograft model of human triple negative breast cancer (TNBC) cell line, MX-1. After tumor growth was confirmed, PTX (20 mg/kg; i.v.) + BEV (5 mg/kg; i.p.) treatment was started (Day 1). Each agent was administered...

Draft of March 11, 2001. We still plan to make considerable changes to this manuscript. Comments and list of typos are welcome!

Context-sensitive points-to analysis is the current most scalable technology for constructing a precise control-flow graph for large object-oriented programs. One appealing feature of this framework is that it is parametric thus allowing to trade time for precision. Typical instances of this framework are k-CFAs and Agesen’s Cartesian Product Algorithm (CPA). It is common sense that k-CFAs (for...

Leishmania mexicana mutants lacking cysteine proteinase genes cpa (delta cpa), cpb (delta cpb), or both cpa and cpb (delta cpa/cpb) have been generated by targeted gene disruption. Delta cpa mutants produce a disease phenotype in BALB/c mice close to that of wild-type L. mexicana, but delta cpb mutants are much less infective, producing very slowly growing small lesions, and delta cpa/cpb doubl...

Cyclopiazonic acid (α-cyclopiazonic acid, α-CPA) is an indole-hydrindane-tetramic acid neurotoxin produced by various fungal species, including the notorious food and feed contaminant Aspergillus flavus. Despite its discovery in A. flavus cultures approximately 40 years ago, its contribution to the A. flavus mycotoxin burden is consistently minimized by our focus on the more potent carcinogenic...

The effect of adenosine on Na+/H+ exchange activity was examined in cultured A6 renal epithelial cells. Adenosine and its analogue N6-cyclopentyladenosine (CPA) had different effects on Na+/H+ exchange activity depending on the side of addition. Basolateral CPA induced a stimulation of Na+/H+ exchange activity that was completely prevented by preincubation with an A2A-selective antagonist, 8-(3...

Implantation of intervertebral disc (IVD) allograft or tissue engineered disc constructs in the spine has emerged as an alternative to artificial disc replacement for the treatment of severe degenerative disc disease (DDD). Establishment of a bank of cryopreserved IVD allografts enables size matching and facilitates logistics for effective clinical management. However, the biomechanical propert...

We have studied the effects of cyclopiazonic acid (CPA) and halothane on the enzymatic activity, oligomeric state, and conformational equilibrium of the Ca-ATPase in skeletal muscle sarcoplasmic reticulum (SR). CPA is a potent inhibitor of Ca-ATPase activity, and this inhibition is competitive with respect to ATP concentration. Time-resolved phosphorescence anisotropy was used to detect the fra...