This is the first report on dual synthesis of poly[(R)-3-hydroxybutyric acid] (PHB) and protein polymer by recombinant microorganisms for use in the fabrication of a biofunctional PHB-based material hybridized with functional proteins. PHB and recombinant silk protein, a model protein developed in this study, were synthesized by a recombinant Escherichia coli system, and then films of PHB hybri...

Bacillus megaterium uyuni S29, isolated from the Bolivian salt lake Uyuni, displays a high capability to produce poly[(R)-3-hydroxybutyrate] (PHB) in industrial culture media. In order to analyze the influence of salt on biomass formation and PHB production, cultivations at different NaCl concentrations were carried out according to the salinity conditions of the habitats of the strain's origin...

Pseudomonas sp. RZS1 was isolated from distillery effluent and identified based on phenotypic characters and 16s rRNA sequencing. It accumulated optimum amount (703.79 microg/mg of biomass) of poly-beta-hydroxybutyrate (PHB) under aerobic process of fermentation and 75 microg/mg of biomass under the anaerobic process of fermentation. Aerobic fermentation yielded 9.3-fold more PHB than semi-aero...

Biological wastewater treatment processes under a dynamic regime with respect to carbon substrate can result in microbial storage of internal polymers (e.g., polyhydroxybutyrate (PHB)) and their subsequent utilizations. These storage turnovers play important roles in nitrous oxide (N2O) accumulation during heterotrophic denitrification in biological wastewater treatment. In this work, a mathema...

Poly(3-hydroxybutyrate) (PHB) depolymerase from Alcaligenes faecalis T1 is composed of three domains: the catalytic (C) domain, the fibronectin type III-like (F) domain, and the substrate-binding (S) domain. We constructed domain deletion, inversion, chimera, and extra-F-domain mutants and examined their enzyme activity and PHB-binding ability. In addition, we performed substitution of 214Asp a...

BACKGROUND Petroleum polymers contribute to non-degradable waste materials and it would therefore be desirable to produce ecofriendly degradable materials. Biodegradation of polyhydroxybutyrate (PHB) in the presence of oligomer hydrolase and PHB depolymerase gave 3-hydroxybutyric acid which could be oxidized to acetyl acetate. Several bacteria and fungi can degrade PHB in the soil. RESULTS Bi...

INTRODUCTION Autophagy is an adaptive response to extracellular and intracellular stress by which cytoplasmic components and organelles, including damaged mitochondria, are degraded to promote cell survival and restore cell homeostasis. Certain genes involved in autophagy confer susceptibility to Crohn's disease. Reactive oxygen species and pro-inflammatory cytokines such as tumor necrosis fact...

BACKGROUND Over the last 10-15 years, a technology has been developed to engineer bacterial poly(3-hydroxybutyrate) (PHB) inclusions as functionalized beads, for applications such as vaccines, diagnostics and enzyme immobilization. This has been achieved by translational fusion of foreign proteins to the PHB synthase (PhaC). The respective fusion protein mediates self-assembly of PHB inclusions...

In order to facilitate oxidative addition chemistry of fac-coordinated rhodium(I) and iridium(I) compounds, carbene–bis(oxazolinyl)phenylborate proligands have been synthesized and reacted with organometallic precursors. Two proligands, PhB(OxMe2)2(ImtBuH) (H[1]; OxMe2 = 4,4-dimethyl-2-oxazoline; ImtBuH = 1-tert-butylimidazole) and PhB(OxMe2)2(ImMesH) (H[2]; ImMesH = 1-mesitylimidazole), are de...