The Nef protein of primate immunodeficiency viruses plays an important role in the pathogenesis of acquired immunodeficiency syndrome (AIDS) [1] [2]. The interaction of Nef with the Nef-associated kinase (NAK) is one of the most conserved properties of different human and simian immunodeficiency virus (HIV and SIV) Nef alleles. The role of NAK association is currently not known but it has been ...

We have recently identified the Nef-associated serine-threonine kinase (NAK) as the p21-activated kinase 2 (PAK2). Here we have taken advantage of the possibility to manipulate the functional properties of NAK by transfecting PAK2 cDNA or its mutant derivatives in order to further characterize the Nef-NAK complex. To exclude the possibility that some Nef variants might interact with PAK1 instea...

OBJECTIVE Alkali metal appears to be a promising tool in thermochemical ablation, but, it requires additional data on safety is required. The objective of this study was to explore the effectiveness of permeable oil-packed liquid alkali metal in the thermochemical ablation of tumors. METHODS Permeable oil-packed sodium-potassium (NaK) was prepared using ultrasonic mixing of different ratios o...

Flux-dependent inactivation that arises from functional coupling between the inner gate and the selectivity filter is widespread in ion channels. The structural basis of this coupling has only been well characterized in KcsA. Here we present NMR data demonstrating structural and dynamic coupling between the selectivity filter and intracellular constriction point in the bacterial nonselective ca...

One of the most important problems in reliable multicast communication is reducing generated redundant NAKs to avoid NAK implosion. A number of NAK suppression mechanisms which try to resolve this technical problem have been proposed thus far. In wireless multicast which includes mobile hosts with wireless access channel, packet losses occur because of not only low quality of wireless communica...

Today's extensive use of multicast communication demands efficiency, scalability, and reliability. Numerous schemes have been proposed to improve these critical areas and some progress has been realized. Among the most recent and effective schemes that address this issue include the tree-based NAK suppression scheme that emphasizes on reducing NAK implosion at the repair node. The procedure emp...

In reliable multicast communications, lost information due to packet loss should be re-multicasted. NAK-based retransmission scheme is said to be effective for scalable reliable multicast communications because it can avoid implosion of control packet. When the source sends packets faster than receiver’s capacity, packet loss due to buffer overflow occurs constantly at corresponding receivers. ...

Nef proteins from human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) have been found to associate with an active cellular serine/threonine kinase designated Nef-associated kinase (Nak). The exact identity of Nak remains controversial, with two recent studies indicating that Nak may be either Pak1 or Pak2. In this study, we investigated the hypothesis that such d...