We report the molecular cloning and sequence of the cDNA coding for the biotin-containing subunit of the chloroplastic acetylcoenzyme A (CoA) carboxylase (ACCase) of Arabidopsis thaliana (CAC1). The 3' end of the CAC1 sequence, coding for a peptide of 94 amino acids, which includes a putative biotinylation motif, was expressed in Escherichia coli as a glutathione-S-transferase (GST) fusion prot...

Acetyl-CoA carboxylase (ACCase) catalyses the carboxylation of acetyl-CoA, forming malonyl-CoA, which is used in the plastid for fatty acid synthesis and in the cytosol in various biosynthetic pathways including fatty acid elongation. In Arabidopsis thaliana, ACC1 and ACC2, two genes located in a tandem repeat within a 25-kbp genomic region near the centromere of chromosome 1, encode two multif...

Echinochloa spp. is one of the most invasive weeds in rice fields worldwide. Acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibiting herbicides are two widely used herbicides. However, overuse has led to resistance evolution penoxsulam (ALS-inhibitor) cyhalofop-methyl (ACCase-inhibitor). In this work, 137 different populations were collected Extremadura (western Spain) where ...

Two genes, accB and accE, that form part of the same operon, were cloned from Streptomyces coelicolor A3(2). AccB is homologous to the carboxyl transferase domain of several propionyl coezyme A (CoA) carboxylases and acyl-CoA carboxylases (ACCases) of actinomycete origin, while AccE shows no significant homology to any known protein. Expression of accB and accE in Escherichia coli and subsequen...

All plant cells produce fatty acids from acetyl-CoA by a common pathway localized in plastids. Although the biochemistry of this pathway is now well understood, much less is known about how plants control the very different amounts and types of lipids produced in different tissues. Thus, a central challenge for plant lipid research is to provide a molecular understanding of how plants regulate ...

Sorghum halepense (L.) Pers. is a troublesome weed of maize which reproduces through seeds and rhizomes. Resistance to ACCase-inhibiting herbicides due target-site mutation Ile-Asn in position 2041 the ACCase gene was confirmed several populations Italy. The presence fitness costs related allelic variant Ile2041Asn has never been investigated ACCase-resistant S. . Three growth analyses pot expe...

Chinese sprangletop (Leptochloa chinensis (L.) Nees) is recognized as the most disturbing weed in rice fields Africa, Australia, and Asia due to causing a significant reduction yields. The habit of lowland farmers Indonesia using high doses herbicides more than once without any rotation, leading increased resistance potential. Therefore, this study aimed confirm level L. acetyl-CoA carboxylase ...

Plant seed oil represents a major renewable source of reduced carbon, but little is known about the biochemical regulation of its synthesis. The goal of this research was to identify potential feedback regulation of fatty acid biosynthesis in Brassica napus embryo-derived cell cultures and to characterize both the feedback signals and enzymatic targets of the inhibition. Fatty acids delivered v...