Camptothecin (CPT) derivatives are topoisomerase I (top1) inhibitors recently introduced as clinical agents. To explore the role of p53 in CPT-induced cytotoxicity, we examined CPT effects in two isogenic pairs of human cancer cell lines, MCF-7 breast carcinoma and HCT116 colon carcinoma cells, in which p53 function had been disrupted by transfection with the human papillomavirus type-16 E6 gen...

Camptothecin (CPT) and doxorubicin (DOX) have been demonstrated to have potent anti-tumor activity. The B-cell translocation gene 2 (BTG2) is involved in the regulation of cell cycle progression. We evaluated the molecular mechanisms of CPT and DOX on cell proliferation and the expressions of BTG2 and prostate specific antigen (PSA) in prostate carcinoma cells. Our results indicated that CPT or...

Cryptotanshinone (CPT), isolated from the plant Salvia miltiorrhiza Bunge, is a potential anticancer agent. However, the underlying mechanism remains to be defined. Here, we show that CPT inhibited lymphangiogenesis in an in vitro model (tube formation). This effect was partly attributed to inhibiting expression of VEGF receptor 3 (VEGFR-3) in murine lymphatic endothelial cells (LEC), as overex...

Camptothecin (CPT), a topoisomerase I inhibitor, forms a cleavable complex with topoisomerase I and single-stranded DNA. When this complex meets a replication fork, the collision generates irreversible double-strand breaks, thereby inducing apoptosis. Based on the mechanism of action, we hypothesized that cycling cells would be more sensitive to CPT than non-cycling cells and that cells stimula...

Introduction Conventional Chest Physical Therapy Directed Cough and Forced Expirations Postural Drainage Chest Percussion Other Airway Clearance Techniques High-Frequency Chest Wall Compression Positive Expiratory Pressure Therapy Autogenic Drainage Exercise Vibratory PEP Therapy Identifying Patients Who Will Benefit From CPT Selecting and Applying CPT Components Promoting Patient Adherence to ...

The properties of two carnitine acyltransferases (CPT) purified from bovine liver are compared to confirm that they are different proteins. The soluble CPT and the inner CPT from mitochondria differ in subunit Mr, native Mr, pI and reactivity with thiol reagents. All eight free thiol groups in soluble CPT react with 5,5'dithiobis-(2-nitrobenzoate) in the absence of any unfolding reagent, and ac...

One of the most fundamental symmetries in physics is CPT invariance. This article reviews the conditions under which CPT symmetry holds by recalling two proofs of the CPT theorem: The original Lagrangian-based analysis and the more rigorous one in the context of axiomatic quantum field theory. The presentation of the proofs is followed by a discussion of the major physical implications that ari...

OBJECTIVES The cold pressor task (CPT) is an experimental method of inducing pain. Ethical concerns have been raised regarding the nontherapeutic induction of pain in children. The objectives of this study were to describe the ethical challenges and acceptability of the CPT from the perspective of researchers, children and parents. METHODS Study 1:16 researchers completed a survey regarding t...

Carnitine palmitoyltransferase (CPT)-I catalyses the transfer of long-chain fatty acids from CoA to carnitine for translocation across the mitochondrial inner membrane. Expression of the 'liver' isoform of the CPT-I gene (CPT-Ialpha) is subject to developmental, hormonal and tissue-specific regulation. To understand the basis for control of CPT-Ialpha gene expression, we have characterized the ...

CPT-AX performance deficit in schizophrenia is well documented but it is unclear what causes this impairment. Past studies have focused on the roles of sustained attention and context processing in CPT-AX but the role of working memory (WM) has not been fully examined even though encoding and maintenance of the cue in WM may be critical to CPT-AX. The major goal of this study was to investigate...