It has been conjectured that any partial triple system of order u and index λ can be embedded in a triple system of order v and index λ whenever v ≥ 2u + 1, λ(v − 1) is even and λ ( v 2 ) ≡ 0 (mod 3). This conjecture is known to hold for λ = 1 and for all even λ ≥ 2. Here the conjecture is proven for all remaining values of λ when u ≥ 28.

UNLABELLED Recombineering, in vivo genetic engineering with bacteriophage homologous recombination systems, is a powerful technique for making genetic modifications in bacteria. Two systems widely used in Escherichia coli are the Red system from phage λ and RecET from the defective Rac prophage. We investigated the in vivo dependence of recombineering on DNA replication of the recombining subst...

Seagrass meadows provide crucial ecosystem services to the coastal zone but are threatened globally. Seagrass loss to date has mainly been attributed to anthropogenic activities that reduce light quantity (amount of photosynthetic photon flux density), such as dredging, flooding and eutrophication. However, light quality (wavelengths of light within the visible spectrum) is also altered by thes...

The traditional banking functions of lending, deposit-taking and payment intermediation are being unbundled in the new frontiers money that extend from virtual currencies to crypto-assets shadow payments quasi-money. possibility for digital-centred change financial industry is illustrated by distributed ledger technology, which ‘blockchain’ most prominent example automated decision-making. Othe...

The notion of conflict disruption is proposed as an addition to the established response framework management, resolution and transformation. Drawing on Schumpeter’s idea creative disruption, article considers how disruptive actions or stances may trigger operate within resolution, transformation Moreover, prompts us think peace in systemic terms: peaceandconflict. Thus concludes by discussing ...

In this paper, we use the type Ia supernova data to constrain the model of holographic dark energy. For d = 1, the best fit result is Ω0m = 0.25, the equation of the state of the holographic dark energy w Λ = −0.91 and the transition between the decelerating expansion and the accelerating expansion happened when the cosmological red-shift was zT = 0.72. If we set d as a free parameter, the best...

The genetic modification of primary bacterial disease isolates is challenging due to the lack of highly efficient genetic tools. Herein we describe the development of a modified PCR-based, λ Red-mediated recombineering system for efficient deletion of genes in Gram-negative bacteria. A series of conjugally transferrable plasmids were constructed by cloning an oriT sequence and different antibio...