نتایج جستجو برای: xylose

تعداد نتایج: 4460  

2015
Biao Zhang Jia Zhang Dongmei Wang Xiaolian Gao Lianhong Sun Jiong Hong

Conversion of xylose to ethanol by yeasts is a challenge because of the redox imbalances under oxygen-limited conditions. The thermotolerant yeast Kluyveromyces marxianus grows well with xylose as a carbon source at elevated temperatures, but its xylose fermentation ability is weak. In this study, a combination of the NADPH-preferring xylose reductase (XR) from Neurospora crassa and the NADP(+)...

2013
Liuyang Diao Yingmiao Liu Fenghui Qian Junjie Yang Yu Jiang Sheng Yang

BACKGROUND It remains a challenge for recombinant S. cerevisiae to convert xylose in lignocellulosic biomass hydrolysates to ethanol. Although industrial diploid strains are more robust compared to laboratory haploid strains, however, industrial diploid S. cerevisiae strains have been less pursued in previous studies. This work aims to construct fast xylose-fermenting yeast using an industrial ...

2016
Trey K Sato Mary Tremaine Lucas S Parreiras Alexander S Hebert Kevin S Myers Alan J Higbee Maria Sardi Sean J McIlwain Irene M Ong Rebecca J Breuer Ragothaman Avanasi Narasimhan Mick A McGee Quinn Dickinson Alex La Reau Dan Xie Mingyuan Tian Jennifer L Reed Yaoping Zhang Joshua J Coon Chris Todd Hittinger Audrey P Gasch Robert Landick

The inability of native Saccharomyces cerevisiae to convert xylose from plant biomass into biofuels remains a major challenge for the production of renewable bioenergy. Despite extensive knowledge of the regulatory networks controlling carbon metabolism in yeast, little is known about how to reprogram S. cerevisiae to ferment xylose at rates comparable to glucose. Here we combined genome sequen...

Journal: :Applied and environmental microbiology 1985
J N Nigam R S Ireland A Margaritis M A Lachance

Natural habitats of yeasts were examined for the presence of strains able to produce ethanol from d-xylose. Black knots, insect frass, and tree exudates were screened by enrichment in liquid d-xylose-yeast extract medium. These and each d-xylose-assimilating yeast in a collection from cactus fruits and Drosophila spp. were tested for alcohol production from this sugar. Among the 412 isolates ex...

Journal: :Applied and environmental microbiology 2000
A Eliasson C Christensson C F Wahlbom B Hahn-Hägerdal

For ethanol production from lignocellulose, the fermentation of xylose is an economic necessity. Saccharomyces cerevisiae has been metabolically engineered with a xylose-utilizing pathway. However, the high ethanol yield and productivity seen with glucose have not yet been achieved. To quantitatively analyze metabolic fluxes in recombinant S. cerevisiae during metabolism of xylose-glucose mixtu...

2016
Gabriel M Rodriguez Murtaza Shabbir Hussain Lauren Gambill Difeng Gao Allison Yaguchi Mark Blenner

BACKGROUND The oleaginous yeast, Yarrowia lipolytica, has been utilized as an industrial host for about 60 years for various applications. Recently, the metabolic engineering of this host has become increasingly popular due to its ability to accumulate lipids as well as improvements made toward developing new genetic tools. Y. lipolytica can robustly metabolize glucose, glycerol, and even diffe...

2014
W. Zhang H. Mou H. Cui

As a clean and renewable source of energy, ethanol is a promising alternative to fossil fuels.1 Especially lignocelluloses, as raw material for ethanol production, have gained great interest during these years.2 Production of bio-ethanol will help to cope with the over-consumption of fossil fuels and further work for the reduction of carbon dioxide emissions. Lignocelluloses (crop residue, cell...

Journal: :Metabolic engineering 2013
John Masani Nduko Ken'ichiro Matsumoto Toshihiko Ooi Seiichi Taguchi

Xylose, which is a major constituent of lignocellulosic biomass, was utilized for the production of poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB)], having transparent and flexible properties. The recombinant Escherichia coli JW0885 (pflA(-)) expressing LA-polymerizing enzyme (LPE) and monomer supplying enzymes grown on xylose produced a copolymer having a higher LA fraction (34mol%) than tha...

Journal: :Applied and environmental microbiology 1991
M Posno P T Heuvelmans M J van Giezen B C Lokman R J Leer P H Pouwels

The inability of two Lactobacillus strains to ferment D-xylose was complemented by the introduction of Lactobacillus pentosus genes encoding D-xylose isomerase, D-xylulose kinase, and a D-xylose catabolism regulatory protein. This result opens the possibility of using D-xylose fermentation as a food-grade selection marker for Lactobacillus spp.

Journal: :Biotechnology and bioengineering 1985
N J Alexander

Pachysolen tannophilils is a yeast capable of fermenting xylose to ethanol. I,Z This organism is of interest due to its potential for converting the xylose found in agricultural and forestry residues to ethanol. Hemicellulosic sugars (i.e., xylose) are relatively abundant in certain plant residues and can be isolated with relative ease (for review, see ref. 3). The assumed pathway of xylose uti...

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