We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

This article explores the signi cant role played by religious groups in the legitimation of the Former Shu founded by Wang Jian in the chaotic early tenth century. Whereas the Daoist priest Du Guangting attempted to legitimate the regime on religious grounds, the Buddhist monk Guanxiu employed his literary talent to depict the military ruler as a savior in a tumultuous age. This article also su...

the purpose of the present paper is to survey and analyse the concept and significance of religious tolerance and universal brotherhood in imam ali’s teachings, especially his nahj al balagha. islam admits the freedom of thought, free expression of opinions, and exercise of independent judgment in religious matters. it also acknowledges tolerance among religions, religious communities, nations ...

Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accretion of mass on the sensitive surface of microresonators is monitored via an integrated optical re...

nasir khosraw ghobadiani balkhi, the iranian poet and philosopher in 5th century (hegira) has poetry and prose works and he has employed to teach religious. his poetry is enjoying of divine inspiration and is overfilled of theology and preaching and asceticism. his prose works has written about philosophy issues and religious points and interpretations of religious commandments and worships. he...