While carrying out routine Widal tests at the Pasteur Institute Laboratory, Shillong, we met from time to time with specimens from patients in Assam whose history suggested enteric fever, but whose serum gave Widal reactions not in the least indicative of that disease, even when repeated two or three times at weekly intervals. Whilst this, of course, is a common enough experience in any laborat...

Introduction: Typhoid fever is endemic in many parts of the world and represents a major cause of acute febrile illness (AFI). Rapid and accurate laboratory methods for diagnosis of this disease are needed for both patient care and surveillance situations. Methodology: Serum samples were collected from AFI patients and used to evaluate the performance of a newly developed ELISA assay that uses ...

Estimates for the year 2000 suggested that there were approximately 21.5 million infections and 200,000 deaths from typhoid fever globally each year, making the disease one of the most serious infectious disease threats to public health on a global scale. However, these estimates were based on little data, especially from Africa. Global prominence and high-profile outbreaks have created the per...

INTRODUCTION Typhoid fever is endemic in many parts of the world and represents a major cause of acute febrile illness (AFI). Rapid and accurate laboratory methods for diagnosis of this disease are needed for both patient care and surveillance situations. METHODOLOGY Serum samples were collected from AFI patients and used to evaluate the performance of a newly developed ELISA assay that uses ...

Typhoid is a global problem. Conventional diagnostic methods have limitations. The Widal test gives a high proportion of false positive results, and indiscriminate use of antibiotics has reduced the utility of blood culture. Consequently, these procedures are inadequate for diagnosing suspected cases of typhoid that do not present clear-cut symptoms. We previously showed that PCR-based diagnosi...

Diagnosis of typhoid fever is difficult because it is non-specific symptoms and signs with those of other acute febrile illness. Blood cultures, and antibody levels of O& H, were evaluated, in the diagnosis of typhoid fever in Egyptian hospitals. Patients were divided into three groups: (I) 254 with blood culture-confirmed typhoid, (II) 3426 febrile patients with negative blood cultures, and (I...

OBJECTIVE To evaluate three commercial typhoid rapid antibody tests for Salmonella Typhi antibodies in patients suspected of having typhoid fever in Mpumalanga, South Africa, and Moshi, United Republic of Tanzania. METHODS The diagnostic accuracy of Cromotest(®) (semiquantitative slide agglutination and single tube Widal test), TUBEX(®) and Typhidot(®) was assessed against that of blood cultu...

Background: To study the reliability of a single Widal test and to find out the diagnostic significance of ‘O’ and ‘H’ agglutinin titre in the diagnosis of typhoid fever. Methods: Community-based case-control study conducted from Jan 2001 to June 2007. The blood samples were collected from the medical and out door department of Sir Ganga Ram Hospitals, Lahore. The diagnostic value of an acute p...

PURPOSE To investigate the differences in clinical features and laboratory findings between group D and non-group D non-typhoidal Salmonella (NTS) gastroenteritis in children. METHODS A retrospective chart review of children diagnosed with NTS confirmed by culture study was performed. The clinical features and laboratory findings of group D and non-group D NTS were compared. RESULTS From 20...