نتایج جستجو برای: western blat

تعداد نتایج: 183956  

Journal: :Bioinformatics 2013
Chengjun Zhang Jun Wang Manyuan Long Chuanzhu Fan

SUMMARY gKaKs is a codon-based genome-level Ka/Ks computation pipeline developed and based on programs from four widely used packages: BLAT, BLASTALL (including bl2seq, formatdb and fastacmd), PAML (including codeml and yn00) and KaKs_Calculator (including 10 substitution rate estimation methods). gKaKs can automatically detect and eliminate frameshift mutations and premature stop codons to com...

2014
Anthony J. Travis Jonathan Moody Aleksandra Helwak David Tollervey Grzegorz Kudla

Associations between proteins and RNA-RNA duplexes are important in post-transcriptional regulation of gene expression. The CLASH (Cross-linking, Ligation and Sequencing of Hybrids) technique captures RNA-RNA interactions by physically joining two RNA molecules associated with a protein complex into a single chimeric RNA molecule. These events are relatively rare and considerable effort is need...

2015
Panu Somervuo Liisa Holm

Proteins evolve by mutations and natural selection. The network of sequence similarities is a rich source for mining homologous relationships that inform on protein structure and function. There are many servers available to browse the network of homology relationships but one has to wait up to a minute for results. The SANSparallel webserver provides protein sequence database searches with imm...

Journal: :Statistical applications in genetics and molecular biology 2009
Sylvain Forêt Susan R Wilson Conrad J Burden

Word matches are often used in sequence comparison methods, either as a measure of sequence similarity or in the first search steps of algorithms such as BLAST or BLAT. The D2 statistic is the number of matches of words of k letters between two sequences. Recent advances have been made in the characterization of this statistic and in the approximation of its distribution. Here, these results ar...

2017
Michael Tillich Pascal Lehwark Tommaso Pellizzer Elena S. Ulbricht-Jones Axel Fischer Ralph Bock Stephan Greiner

We have developed the web application GeSeq (https://chlorobox.mpimp-golm.mpg.de/geseq.html) for the rapid and accurate annotation of organellar genome sequences, in particular chloroplast genomes. In contrast to existing tools, GeSeq combines batch processing with a fully customizable reference sequence selection of organellar genome records from NCBI and/or references uploaded by the user. Fo...

Journal: :iranian journal of parasitology 0
b meshgi a eslami a halajian

background: mixed infection with amphistomes seems common in native cattle of iran. the aim of this study was to determine diagnostic antigens in cattle mixed amphistomiasis. methods: specific antigens of cotylophoron cotylophorum , gastrothylax crumenifer and paramphisto­mum cervi (mixed infection), the most common species, were collected from cattle was deter­mined. adult trematodes were coll...

Journal: :iranian journal of virology 0
sf shams mashhad university of medical sciences (mums), mashhad, iran z arianpour blood transfusion organization research center, mashhad, iran f molahosseini-foomani blood transfusion organization research center, mashhad, iran hgh noorin blood transfusion organization research center, mashhad, iran

background and aims: hiv is spreading rapidly among people word wide. infection with this virus leads to immune suppression and finally acquired immune deficiency syndrome (aids).early hiv detection is depended on antibody screening against virus by enzyme linked immunosorbent assay (elisa).some confirmatory tests such as western blot and recombinant immunobloting assay (riba), are used to veri...

Journal: :journal of crop protection 2015
arezu lotfi masoud shams-bakhsh bagher yakhchali

serological methods are commonly used methods for detection of viruses. preparation of pure viral antigens is a crucial step in production of antibodies required for serological studies. in this research the gene encoding coat protein of a beet western yellows virus (bwyv) isolate from iran was amplified by pcr and was ligated into a bacterial expression vector (pet26b) to obtain pet-bwyv-cp cl...

Journal: :Nucleic Acids Research 2006
Angela S. Hinrichs Donna Karolchik Robert Baertsch Galt P. Barber Gill Bejerano Hiram Clawson Mark Diekhans Terrence S. Furey Rachel A. Harte Fan Hsu Jennifer Hillman-Jackson Robert M. Kuhn Jakob Skou Pedersen Andy Pohl Brian J. Raney Kate R. Rosenbloom Adam C. Siepel Kayla E. Smith Charles W. Sugnet A. Sultan-Qurraie Daryl J. Thomas Heather Trumbower R. J. Weber M. Weirauch Ann S. Zweig David Haussler W. James Kent

The University of California Santa Cruz Genome Browser Database (GBD) contains sequence and annotation data for the genomes of about a dozen vertebrate species and several major model organisms. Genome annotations typically include assembly data, sequence composition, genes and gene predictions, mRNA and expressed sequence tag evidence, comparative genomics, regulation, expression and variation...

2010
Xiang-Ping Wang Nigel G.F. Cooper

BACKGROUND Cytoplasmic polyadenylation element binding proteins (Cpebs) are a family of proteins that bind to defined groups of mRNAs and regulate their translation. While Cpebs were originally identified as important features of oocyte maturation, recent interest is due to their prospective roles in neural system plasticity. RESULTS In this study we made use of bioinformatic tools and method...

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