نتایج جستجو برای: watermelon mosaic virus

تعداد نتایج: 411611  

2015
Ghada A. Abu El-Heba Gihan M. Hussein Inas F. Fahmy Sara M. Abdou Asmaa Faisal Omnia Taha Naglaa A. Abdallah

The production of new cultivars via recombinant DNA technology is important in applied agriculture. Promoters play fundamental roles in successful transformation and gene expression. Fragments of the upstream regulatory region of the movement protein gene of the Tomato yellow leaf curl virus (TYLCV; two fragments) and Watermelon chlorotic stunt virus (WmCSV, two fragments) and one fragment of t...

Journal: :Iranian journal of biotechnology 2015
Xueqin Rao Jie Sun

BACKGROUND Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. OBJECTIVES Development of a highly sensitive and reliable detection method for WSMoV. MATERIALS AND METHODS Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...

2017
Nam-Gyu Kim Eun-Young Seo Sang-Hyuk Han Jun-Su Gong Cheol-Nam Park Ho-Seop Park Leslie L Domier John Hammond Hyoun-Sub Lim

Efforts to control viral diseases in crop production include several types of physical or chemical treatments; antiviral extracts of a number of plants have also been examined to inhibit plant viral infection. However, treatments utilizing naturally selected microorganisms with activity against plant viruses are poorly documented. Here we report isolation of a soil inhabiting bacterium, Pseudom...

2015
Chung-Hao Huang Weng-Rong Hsiao Ching-Wen Huang Kuan-Chun Chen Shih-Shun Lin Tsung-Chi Chen Joseph A. J. Raja Hui-Wen Wu Shyi-Dong Yeh Tetsuro Ikegami

The NSs protein of Watermelon silver mottle virus (WSMoV) is the RNA silencing suppressor and pathogenicity determinant. In this study, serial deletion and point-mutation mutagenesis of conserved regions (CR) of NSs protein were performed, and the silencing suppression function was analyzed through agroinfiltration in Nicotiana benthamiana plants. We found two amino acid (aa) residues, H113 and...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه علوم کشاورزی و منابع طبیعی گرگان - دانشکده کشاورزی گنبد 1390

از بین پوتی ویروس های غلات در ایران دو ویروس موزائیک کوتولگی ذرت(maize dwarf mosaic virus,mdmv) و موزائیک جنوبی مرغ (bermuda grass southern mosaic, bgsmv از لحاظ بیولوژیکی، سرولوژیکی و مولکولی شباهت زیادی بهم دارند. در این تحقیق برای تعیین جایگاه تاکسونومیکی دو ویروس و تمایز دقیق آنها از یکدیگر رابطه دگرپادی بررسی شد. این آزمون در قالب 5 تیمار با 5 تکرار ده بوته ای طراحی شد. تیمار ها شامل مایه ...

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