plants are rich source of phenolic compounds (phenolic acids, flavonoids and tannins) that are most important natural antioxidants. in the present study two ultrasonic methods including probe and bath were used for the extraction of phenolic and flavonoid compounds from celtis australis. the effects of different extraction time including 30, 60 and 90 min (bath method) and 5, 10 and 20 min (pro...

Sonoporation is a phenomenon where ultrasound increases cell membrane permeability. As the result, molecules that are otherwise deprived of transport mechanisms can be transported across the cell membrane. Several different experimental exposure systems are described in the literature. Low-frequency ultrasound (<500 kHz) exposure systems can be divided into two groups: systems with the transduc...

Two patients developed rapidly progressive necrotising fasciitis after being stung by a stonefish. Both were given a hot-water bath for pain relief. The hot water may have accelerated bacterial growth and the consequent development of necrotising fasciitis. Vibrio vulnificus was cultured from one patient. It is recommended that patients should receive high dose of oral and intravenous antibioti...

PUVA therapy is widely used for early stage mycosis fungoides. While the efficacy of PUVA with oral 8-methoxypsoralen (8-MOP) is well documented, the use of its topical variation, bath-water PUVA therapy with 8-MOP has not been studied. The purpose of this study was to assess the effect of 8-MOP bath-water PUVA therapy in adult patients with early stage mycosis fungoides. We retrospectively eva...

Correspondence to: Célia Marisa Rizzatti-Barbosa Department of Prosthesis and Periodontology Piracicaba Dental School, State University of Campinas (UNICAMP) Avenida Limeira, 901. Areião CEP: 13414-018 Piracicaba São Paulo Brazil. Phone: 00 55 19 3412 5373 Fax: 00 55 19 3412 5218 E-mail: [email protected] Abstract The aim of this study was to evaluate the influence of polymerization metho...

Radiofrequency-induced hyperthermia (HT) treatments for cancer include conventional capacitive coupling hyperthermia (cCHT) and modulated electro-hyperthermia (mEHT). In this study, we directly compared these methods with regard to in vitro cytotoxicity and mechanisms of action under isothermal conditions. Hepatoma (HepG2) cells were exposed to HT treatment (42°C for 30 min) using mEHT, cCHT or...

In Present work, the main objective is to develop less time consuming protocol for genomic DNA isolation from leaves of Passiflora foetida. Optimized protocol is cost effective, as it avoided use of expensive liquid nitrogen. The important parameters of CTAB buffer composition such as Polyvinylpyrrolidone PVP40000 (without PVP, 1%, 2%, 3.5%, 4.0%, 4.5%, 5.0%), CTAB (w, 1%, 2%, 3%, 4%, 5%), wate...

To the Editor, We write to highlight the failure of an intravenous fluid warming device, HOTLINE HL-290 Blood and Fluid Warmer (Smiths Medical International, Kent, UK), which could have resulted in harm to the patient. Fluid warmer failures are becoming more prevalent, as evidenced by the United Kingdom Medicines and Healthcare Products Regulatory Agency (MHRA) issuing a recall of all Level 1 S...

BACKGROUND Although the therapeutic mechanism of balneotherapy for atopic dermatitis has not been clarified, many atopic patients who visit thermomineral springs have shown clinical improvements. OBJECTIVE This study was aimed to evaluate the immunomodulatory effect of thermomineral water balneotherapy on the atopic dermatitis murine model. METHODS The oxazolone-induced atopic dermatitis mu...