The estrogenic activity of the chemical UV-filters, 4-methylbenzylidene camphor (4-MBC) and octyl methoxy cinnamate (OMC) was investigated in an vivo rainbow trout ( Oncorhynchus mykiss ) assay. Plasma vitellogenin concentrations were quantified by means Enzyme Linked Immuno Sorbent Assay (ELISA) juvenile before after intraperitoneal injection test compounds. Injection 4-MBC on day 0, 3, 6 10 e...

In most insects, vitellogenesis is regulated by juvenile hormone (JH). The recent cloning of the vitellogenin gene in a number of JH-dependent species has allowed the study of the induction of vitellogenin production by JH at a molecular scale. Potential JH response elements have been described in the regulatory regions of vitellogenin and other JHinducible genes. These elements may not only sh...

Comparative sequence analysis of mosquito vitellogenin (Vg) genes was carried out to gain a better understanding of their evolution. The genomic clones of vitellogenin genes were isolated and sequenced from all three subfamilies of the family Culicidae including Culicinae (Aedes aegypti, Ochlerotatus atropalpus, Ae. polynesiensis, Ae. albopictus, Ochlerotatus triseriatus and Culex quinquefascia...

The specific binding of vitellogenin to chicken oocyte membranes was characterized. This major hen serum phospholipoglycoprotein and one of its lower-molecular-weight components, phosvitin, bound to oocyte membranes with KD values of approx. 6 x 10-7 M. The optimum pH for binding was 6.0, the same as the pH of yolk contents. Phosvitin and vitellogenin compete with each other for binding; other ...

A gene library of Xenopus laevis was constructed from embryonic DNA partially digested with restriction endonucleases Hae III and Alu I and joined to the phage lambda Charon 4 cloning vector with EcoRI linkers. Nucleotide sequences from three of the four related vitellogenin genes have been isolated. Two of the genes (called A1 and A2) were isolated in their entirety together with long stretche...

In the liver of oviparous vertebrates vitellogenin gene expression is controlled by estrogen. The nucleotide sequence of the 5' flanking region of the Xenopus laevis vitellogenin genes A1, A2, B1 and B2 has been determined. These sequences have been compared to each other and to the equivalent region of the chicken vitellogenin II and apo-VLDLII genes which are also expressed in the liver in re...

The yolk precursor protein vitellogenin was isolated from plasma of estrogen-treated Xenopus laevis males, and the yolk platelet proteins, lipovitellin and phosvitin, were isolated from oocytes of females previously stimulated with human chorionic gonadoptropin. The molecular weights of the polypeptide chains of vitellogenin and lipovitellin were determined by electrophoresis on sodium dodecyl ...

Fishes were collected at 16 sites within the three major river drainages (Delaware, Susquehanna, and Ohio) of Pennsylvania. Three species were evaluated for biomarkers of estrogenic/antiandrogenic exposure, including plasma vitellogenin and testicular oocytes in male fishes. Smallmouth bass Micropterus dolomieu, white sucker Catostomus commersonii, and redhorse sucker Moxostoma species were col...