herein, we presented the first report on bluetongue (bt) disease in 10 pregnant camels in a herd fromkerman province, iran. all sera samples were tested serologically (agid, c-elisa). we also used the razi-bk cell line, performed primary culture of ovine kidney and inoculated intravenously the embryonatedchicken eggs (ece) to culture and isolate the bt virus. efforts to culture and isolation of...

The sensitivity of the mouse antibody production test with intraperitoneal or intrasplenic inoculation of mice with reovirus type 3, minute virus of mice, lymphocytic choriomeningitis virus, or mouse hepatitis virus was compared with that of direct isolation in cultured cells. The mouse antibody production test for detection of mouse hepatitis virus was significantly more sensitive than virus i...

A method is described for the isolation of virus-resistant mutants of mammalian cells in culture. Cells of a mouse embryo fibroblast line (3T6) were treated with a mutagen and subjected to selection for virus resistance. The cells were infected with a temperature-sensitive mutant of Sindbis virus which was allowed to proceed through one cycle of replication at the permissive temperature. On tra...

Aujeszky’s Disease (AD) is a major pig disease that accounts for devastating economic losses to pig industry. AD is controlled by containment of infected herds, use of vaccines and removal of latently infected animals. In endemic areas, control programmes against AD rely on the use of vaccines that efficiently limit the replication of virulent virus in infected pigs. The presented study investi...

more. Virus yield, which decreased only when the drug was added early in infection, was reduced several 100fold by 80 tLM aphidicolin. Viral inhibition was reversed by the suspension of the infected cells in drug-free medium. DNA synthesis in uninfected cells was reduced about 10-fold by 1 ,uM aphidicolin. In infected cells, aphidicolin concentrations over 10 ,uM were needed to reduce DNA synth...

The incidence of mumps infection in the UK was reduced greatly by vaccination as a component of MMR vaccine, but cases and outbreaks continue to occur. Although in specialised laboratories RT-PCR is available for detection of mumps virus RNA in clinical samples, in routine laboratories virus isolation remains a standard method for diagnosing mumps virus infection. Furthermore, isolation of mump...

We report here the isolation and complete genome sequencing of a large double-stranded DNA virus, Powai Lake megavirus, for the first time from India. The isolation of a large DNA virus with genome size >1 Mb from India further attests to the prevalence of Giant viruses in different environmental niches.

background: hepatitis g virus (gbv-c) is a single strand rnapositive virus and is a member of flaviviridae family. infection with the virus is prevalent add has worldwide distribution. accurate diagnosis of the virus is very important, because of co-infection with other important viruses like hcv, hbv and hiv, it may influence on pathogenesis, disease progression and response to viral therapy. ...

A commercial system for detection of herpes simplex virus (HSV) DNA by in situ hybridization gave positive results on 16 of 17 stored human brain specimens that were positive for HSV on initial testing by virus isolation and immunofluorescence staining, and the hybridization system gave negative results on 13 brain specimens that showed no evidence of HSV by isolation or immunofluorescence stai...

This study was performed in order to determine the distance of isolation for the seed potato production from ware potato in three regions of Ardabil, Namin and Nir. The experiment was carried out in a randomized complete block design (RCBD) with five distances (100, 200, 300, 400 and 500 meters from ware potato) and three replications with Super Elite (SE) class of Agria potato cultivar from wa...