The effects of fucose-containing sulfated polysaccharides, ascophyllan and fucoidan, isolated from the brown alga Ascophyllum nodosum, on the growth of various cell lines (MDCK, Vero, PtK 1 , CHO, HeLa, and XC) were investigated. In a colony formation assay, ascophyllan and fucoidan showed potent cytotoxic effects on Vero and XC cells, while other cell lines were relatively resistant to these p...

MicroRNAs (miRNAs) are small noncoding RNAs that negatively regulate gene expression post-transcriptionally. They play a critical role in developmental and physiological processes and have been implicated in the pathogenesis of several diseases including cancer. To identify miRNA signatures associated with different stages of neoplastic development, we examined the expression profile of 776 pri...

During one year study, 394 stool samples obtained from a random cases of diarrhea were examined for the presence of free shiga-like toxins Stx1 and Stx2 in Vero cells assay. Of 394 stool specimen supernatants tested, 2 (0.5%) gave positive results. The two stool supernatants positive on Vero cell line were wholly capable of being neutralized with the monoclonal antibody to Stx2. Broth cultures ...

background: prostate cancer is one of the leading causes of cancer deaths among men. new gene expressed in prostate (ngep), is a prostate-specific gene expressed only in normal prostate and prostate cancer tissue. because of its selective expression in prostate cancer cell surface, ngep is a potential immunotherapeutic target. to target the ngep in prostate cancer, it is essential to investigat...

We developed and characterized a stable Vero cell line constitutively expressing Peste des petits ruminants virus (PPRV) hemagglutinin (H) protein and assessed its potential use as diagnostic antigen in enzyme-linked immunosorbent assay (ELISA). PPRV H gene of the vaccine strain (Sungri-96) was amplified by reverse transcription (RT)-PCR, cloned into a eukaryotic expression vector (pTarget), an...

background: this study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (tcm199)–vero cells and menezo b2 (b2)-vero cells for the in vitro developmental quantity and quality of bovine embryos. materials and methods: bovine oocytes were allowed to mature and subsequently undergo fertilization in vitro. their presump...

A genetically modified cell line (Vero-ICP10-EGFP) was constructed for detection of herpes simplex virus (HSV) by a simple, rapid and direct method. The cell line was developed by stable transfection of Vero cell with a plasmid encoding the green fluorescent protein (GFP) driven by the promoter of the HSV-2 ICP10 gene. As early as 6 h after infection with HSV, fluorescence-emitting cells can be...

shiga toxins (stxs) are bacterial virulence factors produced by shigella dysenteriae serotype 1 and escherichia coli strains. stxs are critical factors for the development of diseases such as severe bloody diarrhea and hemolytic uremic syndrome. additionally, stxs trigger the secretion of pro- inflammatory cytokines and chemokines, particularly in monocytes or macrophages. the inflammatory cyto...

background and objectives : each year, enteroviruses infect millions of people and cause different diseases. the agents are usually detected using cell culture. rd (rhabdomyosarcoma) and l20b (l cells) are among the recommended cells by the world health organisation (who) for this purpose. even though cell culture is the most common method used in diagnosing enteroviruses in stool specimens, th...