Purpose: To identify protein targets in host (vero) cell since there is currently no therapy or a licensed tetravalent vaccine to combat all the four virus serotypes of dengue virus. Methods: The domain III of the dengue virus encoded envelope protein was expressed in pET28a expression vector and the purified recombinant protein was labeled with biotin without altering its immunogenicity. Vero ...

Recent studies have suggested that the hydrosalpinx has a negative effect on pregnancy outcome, with markedly diminished implantation and increased early pregnancy loss. Fluid from the hydrosalpinx may leak into and accumulate in the uterine cavity. It is not clear, however if this creates a hostile local environment in the uterus for embryo implantation or exerts a direct embryotoxic effect. T...

Renal disorders have become very common nowadays, which may also lead to kidney failure. The disorder may be caused by the commonly used chemicals such as acetaminophen, CCl4, streptromycin, H2O2 etc. The objective of this work is to determine the nephroprotective potential of ethanolic extract against H2O2 induced toxicity in VERO cell line. Ethanolic extract is known for its antioxidant, anti...

We have previously shown that canine signaling lymphocyte activation molecule (SLAM; also known as CD150) acts as a cellular receptor for canine distemper virus (CDV). In this study, we established Vero cells stably expressing canine SLAM (Vero.DogSLAMtag cells). Viruses were isolated in Vero.DogSLAMtag cells one day after inoculation with spleen samples from five out of seven dogs with distemp...

Clostridium perfringens iota-toxin consists of two separate proteins identified as a cell binding protein, iota b (Ib), which forms high-molecular-weight complexes on cells generating Na(+)/K(+)-permeable pores through which iota a (Ia), an ADP-ribosyltransferase, presumably enters the cytosol. Identity of the cell receptor and membrane domains involved in Ib binding, oligomer formation, and in...

introduction and aims: omeprazole is a gastric parietal cells proton pump inhibitor that is also active against h. pylori in vitro. this study was designed to examine the neutralization of h. pylori cytotoxicity on vero cells by omeprazole micronized in strains isolated from gastritis, ulcer, cancer and barrett's ulcer, to determine whether omeprazole can inhibit vacuolation of the vero cells i...

objective: this study was carried out to examine the effect of vero cells co-culture on developmental competence of immature oocytes. materials and methods: bovine cumulus-oocyte-complexes (cocs) were matured in either presence or absence of vero cells. matured oocytes were inseminated and cultured for up to nine days. cleavage percentages were recorded on day two post insemination (pi). embryo...

The role of the cell nucleus in the development of African swine fever virus in Vero cells has been studied. No viral growth could be detected in enucleated cells under conditions that allow normal development of Sindbis virus. Furthermore, African swine fever virus DNA synthesis was inhibited more than 95% after infection of enucleated Vero cells as compared with normal cells.

Introduction: Inactivated influenza vaccines are traditionally produced in chicken embryonated eggs but its limitations in producing the required doses in pandemic outbreaks quickly enough has made searching for alternative modes of production necessary. The use of cell culture-based vaccine production is one way of overcoming the limitations of the egg-based method and securing a more rapid re...