Due to the relatively large availability and reduced price, the mineral transformer oils are widely used as electrical insulating liquids. However, mineral oil drastically degrades over time in service. New efforts were made to improve mineral oils characteristics, and other types of liquids like vegetal oils are proposed. This paper deals with new comparative tests on mineral and vegetal oils ...

Cytoplasmic RNA localization is a key biological strategy for establishing polarity in a variety of organisms and cell types. However, the mechanisms that control directionality during asymmetric RNA transport are not yet clear. To gain insight into this crucial process, we have analyzed the molecular machinery directing polarized transport of RNA to the vegetal cortex in Xenopus oocytes. Using...

In the first polar lobe of the egg of Bithynia tentaculata a cup-shaped mass of small vesicles is described, which fills the greater part of the lobe. It is named the 'vegetal body'. With methyl green-pyronin the vegetal body stains clearly, but after treatment with RNase no staining occurs, thus indicating the presence of RNA. The first polar lobe of Bithynia is of great importance for further...

qPCR tomography was developed to study mRNA localization in complex biological samples that are embedded and cryo-sectioned. After total RNA extraction and reverse transcription, the spatial profiles of mRNAs and other functional RNAs were determined by qPCR. The Xenopus laevis oocyte was selected as model, because of its large size (more than 1mm) and large amount of total RNA (approximately 5...

Ultra-violet (u.v.) irradiation of the vegetal pole of newly fertilized eggs has three documented effects: reduction of primordial germ cells (PGCs), cytological damage to the vegetal hemisphere and disruption of the normal mechanism by which the vegetal yolk mass induces the formation of the dorsal axis of the embryo. In this study, we find that 90 degrees rotation of the egg for various perio...

The subcellular localization of specific mRNAs is a widespread mechanism for regulating gene expression. In Xenopus oocytes microtubules are required for localization of Vg1 mRNA to the vegetal cortex during the late RNA localization pathway. The factors that mediate microtubule-based RNA transport during the late pathway have been elusive. Here we show that heterotrimeric kinesin II becomes en...

Marijuana is the illicit drug most commonly used worldwide. The mechanisms by which this drug affects the brain have been studied exhaustively during the last 40 years, and better understood in the last decade. For example, the discovery of receptors to which marijuana binds, has been a major achievement in neurosciences and in the study of drug addiction. Moreover, the description of the endog...

During oogenesis, hundreds of maternal RNAs are selectively localized to the animal or vegetal pole, including determinants of somatic and germline fates. Although microarray analysis has identified localized determinants, it is not comprehensive and is limited to known transcripts. Here, we utilized high-throughput RNA-sequencing analysis to comprehensively interrogate animal and vegetal pole ...

BACKGROUND The vegetal biomembrane has been used to treat cutaneous ulcers. OBJECTIVES To assess the role of the vegetal biomembrane on the chronic venous ulcers treatment compared to treatment with collagenase cream. METHODS Fourteen patients were selected to be treated with vegetal biomembrane and 7 with Fibrase(®) (CONTROL), followed clinically and photographically by the Wound Healing I...

In zebrafish, as in many animals, maternal dorsal determinants are vegetally localized in the egg and are transported after fertilization in a microtubule-dependent manner. However, the organization of early microtubules, their dynamics and their contribution to axis formation are not fully understood. Using live imaging, we identified two populations of microtubules, perpendicular bundles and ...