Norton, J. E. (University of Oklahoma School of Medicine, Oklahoma City), and J. R. Sokatch. Oxidation of d- and l-valine by enzymes of Pseudomonas aeruginosa. J. Bacteriol. 92:116-120. 1966.-Cell-free extracts prepared from Pseudomonas aeruginosa grown on dl-valine catalyzed the consumption of oxygen with several d-amino acids, but not with the corresponding l-amino acids. The product of d-val...

The direct influence of glucagon on rates of valine incorporation into and release from protein was assessed in the perfused rat liver. Glucagon alone significantly increased net valine release, an effect which was ascribed largely to the stimulation of proteolysis. The magnitude of this increase in absolute terms remained virtually unchanged in the presence of an amino acid mixture that suppre...

Pea (Pisum sativum L. cv Alaska) root tips were excised and cultured aseptically in White's medium. Cultures were treated immediately or after a 24 hour equilibration time with 28 nanomolar chlorsulfuron plus isoleucine and valine (each 0.1 millimolar), isoleucine and valine, or untreated. The percentage of mitotic figures in untreated control roots sampled immediately after excision showed a t...

Shelton, Damon C. (West Virginia University Medical Center, Morgantown), and William E. Nutter. Uptake of valine and glycylvaline by Leuconostoc mesenteroides. J. Bacteriol. 88:1175-1184. 1964.-Uptake systems for valine and glycylvaline were studied in growing cultures and non-growing cellular suspensions of Leuconostoc mesenteroides. Glycylvaline promoted growth more rapidly than did valine. O...

Previous information on the catabolism of valine has been obtained by taking advantage, for the most part, of the gluconeogenic property of valine and using glucose as an indicator of its intermediary metabolism. Thus, Rose et al. (1) reported that valine and a-ketoisovaleric acid have similar gluconeogenic effects, both contributing 3 of their carbon atoms to glucose formation in phlorizinized...

We previously demonstrated efficient L-valine production by metabolically engineered Corynebacterium glutamicum under oxygen deprivation. To achieve the high productivity, a NADH/NADPH cofactor imbalance during the synthesis of l-valine was overcome by engineering NAD-preferring mutant acetohydroxy acid isomeroreductase (AHAIR) and using NAD-specific leucine dehydrogenase from Lysinibacillus sp...

A spontaneous leu-linked mutation (ilvH2015) in Escherichia coli K-12 made the strain resistant to 1 mM valine and l mM glycylvaline (Val-r) and caused the isoleucine and valine biosynthetic enzyme, acetohydroxy acid synthase, to be less sensitive to feedback inhibition by valine than the wild-type enzyme. Transfer of the ilvDAC deletion into a strain carrying ilvH2015 abolished the effect of t...

13C-direct detection NMR has several advantages compared to proton detection, including a tendency relax slower and wider chemical shift range. However, the sensitivity of is much lower than that because its gyromagnetic ratio. In addition, virtual decoupling procedure often performed remove peak splitting in axis, which further reduces 1/√2. this study, enhance experiments, we developed HCACO-...

On the basis of isotopic results obtained with Torulopsis utilis and Neurospora crassa, Strassman et al. (1) and Adelberg (2) have proposed a mechanism for valine biosynthesis in which pyruvate is converted to a-acetolactate. Acetolactate would then undergo a rearrangement to yield the carbon skeleton of valine. Abelson (3) showed that pyruvate, in competition with glucose, gave rise to the car...