We established a typing system for Prevotella intermedia and Prevotella nigrescens using the combination of PCR ribotyping and arbitrarily primed PCR (AP-PCR) fingerprinting and applied this system to the study of intrafamilial incidence of these species in the oral cavity. PCR ribotyping followed by subtyping by AP-PCR fingerprinting was applied to each type strain of P. intermedia and P. nigr...

This cross-sectional study was carried out to identify A. actinomycetemcomitans and P. intermedia in the subgingival plaque of three ethnic groups (Malays, Chinese and Indians) in a selected group of adult Malaysians with advanced Chronic Periodontitis and to correlate these findings with their periodontal status. Thirty periodontally diseased adults were age, gender and ethnically matched with...

In this study, the essential oil (EO) constituents from the aerial parts of Satureja intermedia C.A.Mey were detected by GC and GC/MS. The antimicrobial activity of EO on oral pathogens and its cytotoxicity to human cancer cells were determined by the microbroth dilution method and the crystal violet staining method, respectively. Thirty-nine compounds were identified and the main EO constituen...

Recent evidence suggests that certain periodontal pathogens preferentially stimulate T cells expressing specific variable regions on the beta chain (Vbeta) of the T-cell receptor, which may indicate the presence of a superantigen. Superantigens are microbial proteins that activate large numbers of CD4(+) T cells in a Vbeta-specific manner. The purpose of this study was to determine whether Prev...

Invasion of oral epithelial cells by pathogenic oral bacteria may represent an important virulence factor in the progression of periodontal disease. Here we report that a clinical isolate of Prevotella intermedia, strain 17, was found to invade a human oral epithelial cell line (KB), whereas P. intermedia 27, another clinical isolate, and P. intermedia 25611, the type strain, were not found to ...

PURPOSE Nitric oxide (NO) is a short-lived bioactive molecule that is known to play an important role in the pathogenesis of periodontal disease. In the current study, we investigated the effect of the flavonoid quercetin on the production of NO in murine macrophages activated with lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen related to inflammatory periodontal disease, and t...

Introduction. Patients with β-thalassemia intermedia have a higher incidence of thromboembolic events compared to the general population. Previous studies have shown that patients with sickle cell disease, who are also prone to ischemic events, have higher intracranial arterial blood flow velocities measured by transcranial Doppler sonography (TCD). The aim of this study is to evaluate intracra...

The cellular origins of corticotropin and melanotropin were studied by observing the response of the rat hypophysis to adrenalectomy and cortisol administration when stained immunochemically with peroxidase-labeled antibody following application of antiserums to P1-24-corticotropin, pp17-39-corticotropin, human p-melanotropin, and a-melanotropin. The presence of corticotropin in the pars interm...

Plant remedies, despite their innocuity reputation, are implicated in adverse events. The present review focuses on 30 popular herbs which are associated with severe and sometimes fatal hepatotoxicity: Actaea racemosa, Aloe barbadensis, Atractylis gummifera, Callilepsis laureola, Camellia sinensis, Centella asiatica, Chelidonium majus, Ephedra sinica, Garcinia cambogia, Hypericum perforatum, La...