نتایج جستجو برای: using qrt
تعداد نتایج: 3392536 فیلتر نتایج به سال:
در این تحقیق اثر غلظت های مختلف سیلیکون (صفر، 2، 4 و 6 میلی مولار) بر بیان چند ژن مرتبط با بیماریزایی و فعالیت تعدادی آنزیم آنتی اکسیدان مطالعه شد. گیاهان در مرحلة خوشه دهی از طریق محلول خاک تیمار شدند. سپس، گیاهان تیمار شده با 1 میلی لیتر سوسپانسیون اسپور قارچ حاوی 106 ماکروکنیدی به روش تزریق در زیر محل خوشه، مایه زنی شدند و در بازه های زمانی صفر، 24، 72 و 120 ساعت پس از تلقیح، برداشت نمونه بر...
BACKGROUND Bivalves comprise around 30,000 extant species and have received much attention for their importance in ecosystems, aquaculture and evolutionary studies. Despite the increasing application of real-time quantitative reverse transcription PCR (qRT-PCR) in gene expression studies on bivalve species, little research has been conducted on reference gene selection which is critical for rel...
It is challenging to find genes with stable transcripts for use as reference genes for quantitative realtime polymerase chain reaction (qRT-PCR) during viral infection. Autographa californica nucleopolyhedrovirus (AcMNPV) is known to globally shut off host gene transcription in Sf21 cells and to modify their cytoskeletons. In this study, seven host genes were selected for validation as referenc...
AIM To investigate the dose-response effect of humic acid (HA) on the quantitative real time polymerase chain reaction (QRT-PCR) inhibition and the efficiency of Taq polymerase increment in preventing inhibition by HA in DNA extracted from ancient bones. METHODS DNA was isolated from bone samples and DNA quantification was conducted with the real-time 5' exonuclease detection assay (TaqMan), ...
qRT-PCR is a generally acknowledged method for gene expression analysis due to its precision and reproducibility. However, it is well known that the accuracy of qRT-PCR data varies greatly depending on the experimental design and data analysis. Recently, a set of guidelines has been proposed that aims to improve the reliability of qRT-PCR. However, there are additional factors that have not bee...
We construct 9-parameter and 13-parameter dynamical systems of the plane which map bi-quadratic curves to other bi-quadratic curves and return to the original curve after two iterations. These generalize the QRT maps which map each such curve to itself. The new families of maps include those that were found as reductions of integrable lattices by [11].
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