Methods for extracting PCR-amplifiable DNA from the spores of rusts and other plant pathogenic, obligately parasitic fungi are hindered by small cell size and thick spore walls that make uniform disruption or digestion difficult and by the abundant pigments, oils, protein, and RNA that can interfere with the subsequent purification and PCR amplification (6,14). For some rusts for which abundant...

introduction: in different investigations, the destructive effect of ultrasonic scalers on the margins of composites has been studied, but there are very few studies on the effects of ultrasonic scalers on the microleakage of poly-acid modified composite resin and resin modified glass ionomers. the main purpose of this study was to evaluate the marginal microleakage of class v preparations rest...

A lamb digestion trial was conducted to compare the ability of internal markers to predict digestibility of alfalfa. Lambs were fed alfalfa hay grown with varying amounts of water stress where water per harvest ranged from 10 to 20 cm/ha and yield ranged 1400 to 4200 kg/ha. In vivo dry matter digestibility was most highly correlated with digestibility determined using acid detergent fiber insol...

the eucapnic hyperventilation of cold dry air. The precise mechanism of this effect, including Background – Leukotrienes have been imthe role of biochemically active mediators, is plicated in the mediation of airway obunknown. The cysteinyl leukotrienes (LTC4, struction induced by hyperventilation of LTD4, and LTE4) are potent mediators of cold dry air in asthmatic subjects. The bronchoconstric...

OBJECTIVES To analyse the collagen composition of normal adult human supraspinatus tendon and to compare with: (1) a flexor tendon (the common biceps tendon) which is rarely involved in any degenerative pathology; (2) degenerate tendons from patients with chronic rotator cuff tendinitis. METHODS Total collagen content, collagen solubility and collagen type were investigated by hydroxyproline ...

We developed a rapid acid-digestion method for preparing tissue samples for iron determination. Specimens were digested in nitric acid and hydrogen peroxide under high temperature and pressure in closed Teflon vessels, with microwave energy. Analysis for iron in 25- to 250-mg portions of digested bovine liver powder (National Bureau of Standards Certified Reference Material no. 1577a) showed ex...

Because of the complexity of the interactions involved very few attempts have been made to trace the fate of ingested food in the course of its digestion and absorption along the alimentary tract of the ruminant. Elsden, Hitchcock, Marshall & Phillipson (1946) made a comparative study of the weights of the digesta and their dry-matter and volatile fatty-acid content in the consecutive parts of ...

Cow dung is generally used as the feedstock material for anaerobic digestion to produce biogas. A selection of alternate biomass needed reduce consumption or eliminate use cow dung. Recently, cottonseed hull has been considered primary substrate In this paper, effect biogas production on co-digestion CD with pre-treated CSH using sulfuric acid, hydrochloric hydrogen per oxide, and acetic acid i...

In a 148-d feedlot experiment, 48 crossbred steers were fed a 70% sorghum diet with clinoptilolite (CLN), a natural zeolite, substituted at 0, 1.25 and 2.5% of the diet dry matter. No differences (P>.05) were detected in average daily gain, feed intake (dry matter and organic matter) and feed efficiency among treatments. Negative correlations (P<.05) between fecal starch and fecal pH were detec...