new series of spiroindeno[1,2-b]pyrido[2,3-d]pyrimidine-5,3′-indolines as new urease inhibitors were synthesized by the catalytic procedure in high yield and short reaction time. in this method, biacidic carbon was prepared as a novel heterogeneous acid and was subsequently used as an efficient catalyst in this synthesis. the inhibitory activities of synthesized compounds were tested against ja...

The propert ies of rumen urease were studied. In the absence of high concentrations of dithiothreitol enzyme activity was rapidly lost; in its presence, the enzyme could be solubilized and purified in high yield. Maximum activity was observed between pH 7 and 8.5. The Km for urea with partially purified enzyme was .004 M with Vmax of 300 to 400 #moles/mg/hr. No divalent metal ion requirement or...

The ulcer is a vital disease that unfavorably affects human health globally. Helicobacter pylori, reason forhave an antibacterial effect against ulcers and some stomach disorders, live on using the urease enzyme induces condition to occur. Metal coordination complexes (MCC) are used in numerous industrial areas field. Cyanide bridged metal also applied several places. In current study, characte...

Abstract The identification of novel anti-infective agents synthetic and natural origin represents one the main aims contemporary drug discovery. In current work, four different varieties Nicotiana tabacum , namely, K399, SPG28, Swat No. 1, 2, were studied to assess antibacterial antifungal properties their extracts. extracts contain anthraquinones, alkaloids, saponins, terpenoids, tannins, res...

Urease enzyme has a crucial role in the persistent habitation of Helicobacter pylori (H. pylori) that induces gastrointestinal diseases, in particular gastritis, duodenal, peptic ulcer, and gastric cancer. Plants have long been utilized as the biggest source of substances with medicinal properties from natural origin and therefore result in less toxicity and adverse side effects upon usage. 15 ...

Urease, a nickel-containing metalloenzyme is getting remarkable attention due to diverse range of applications for mankind. Urease plays magnificent role in various field like agriculture, analytical, geological phenomena, beverage industry and an important diagnostic tool. mainly present bacteria, fungi, plants invertebrates its manifestation specific genera may open new vistas taxonomic posit...

The aim of this study was to investigate the chemical composition essential oils (EOs) from two Diplophyllum species and evaluate their bioactivity potential [antimicrobial, antioxidant, anti-urease, anti-xanthine oxidase (XO)], phenolic compounds. analysis albicans taxifolium permitted identification 62 components, comprising ? 99.6% total EO composition. major components found in these liverw...

This study investigates the inhibitory activities of gossypol, a natural polyphenolic compound from Gossypium spp., against Helicobacter pylori (HP) clinical strains and urease enzyme that plays key role in pathogenesis HP. Gossypol was detected to exhibit bacteriostatic action all HP tested with minimum concentration (MIC) values ranging 3.51 4.14 µg/mL. The activity (HPU) efficiently impeded ...

A pH-variation study of jack bean (Canavalia ensiformis) urease steady-state kinetic parameters and of the inhibition constant of boric acid, a urease competitive inhibitor, was performed using both noninhibitory organic (MES, HEPES and CHES) and inhibitory inorganic (phosphate) buffers, in an effort to elucidate the functions exercised in the catalysis by the ionizable groups of the enzyme act...