The inhibition of urease from Sporosarcina pasteurii (SPU) and Canavalia ensiformis (jack bean, JBU) by a class six aromatic poly-hydroxylated molecules, namely mono- dimethyl-substituted catechols, was investigated on the basis inhibitory efficiency catechol scaffold. aim to probe key step mechanism proposed for SPU catechol, sulfanyl radical attack ring, as well obtain critical information ef...

A series of eighteen 1,3,4-oxadiazole derivatives have been synthesized by treating aromatic acid hydrazides with carbon disulfide in ethanolic potassium hydroxide yielding potassium salts of 1,3,4-oxadiazoles. Upon neutralization with 1 N hydrochloric acid yielded crude crystals of 1,3,4-oxadiazoles, which were purified by recrystallization in boiling methanol. The synthesized 1,3,4-oxadiazole...

Foliar urea application on barley plants increased leaf urease activity for 5 hours with a peak of 20-fold at 2 hours. To discern the mode of urease induction, urea with or without inhibitors and [35S]methionine were incubated with leaf sections for different lengths of time. Urease was extracted, partially purified, electrophoresed, and then quantified by fluorogram. Five urease (U) isozymes w...

Infection with Helicobacter pyloriis the most common cause of stomach and duodenal ulcers. About more than 80 % of people are infected with H. pylori in developing countries. H. pylori uses urease enzyme product "ammonia" in order to neutralize and protect itself from the stomach acidic condition and urease enzyme activity has been shown to be essential to the colonization of H. pylori. Inhibit...

The fluidised bed drying characteristics of soybeans at high temperatures (110-140 degrees C) and moisture contents, 31-49% dry basis, were modelled using drying equations from the literature. Air speeds of 2.4-4.1 m/s and bed depths from 10 to 15 cm were used. The minimum fluidised bed velocity was 1.9 m/s. From a quality point of view, fluidised bed drying was found to reduce the level of ure...

BACKGROUND The inhibitory effect of andrographolide sodium bisulphite (ASB) on jack bean urease (JBU) and Helicobacter pylori urease (HPU) was performed to elucidate the inhibitory potency, kinetics and mechanism of inhibition in 20 mM phosphate buffer, pH 7.0, 2 mM EDTA, 25 °C. METHODS The ammonia formations, indicator of urease activity, were examined using modified spectrophotometric Berth...

A homology model of Helicobacter pylori urease was developed by using the crystal structure of urease from Klebsiella aerogenes (EC 3.5.1.5) as a template. The acetohydroxamic acid moiety was docked into the active pocket of the enzyme model, followed by relaxation of the complex by use of molecular dynamics. The resulting conformation was used as a template to construct 24 potential dipeptide ...

Water-soluble compounds (WS) obtained from the pyrolysis of three lignocellulosic biomasses (larch, poplar, and switchgrass) were tested as potential inhibitors enzyme urease. Thanks to presence an array phenolic like catechol, methoxy/hydroxy phenols, acids, aldehydes, all WS samples at a catechol concentration 30 μM inhibited activity jack bean urease (JBU) by 60%–70% 80% that naturally prese...