Objectives: The present article involved the development of sensitive and validated Ultraviolet (UV) Spectrophotometric reverse phase liquid chromatographic method for determination lapatinib in bulk pharmaceutical dosage form. Materials Methods: UV is based on measurement absorption at maximum 268 nm using methanol as a solvent. In High Performance Liquid Chromatography (HPLC) method, Waters H...

Submit Manuscript | http://medcraveonline.com HPLC-UV: High Performance Liquid Chromatography And Ultravioleta Detector; HPLC-DAD: High Performance Liquid Chromatography and Diode Array Detector; NCB: Blank Nanocapsules; NCLS: Nanocapsules Containing Essential Oil of L. Sidoides; ODS: Octadecylsilyl Groups; OELS: Essential Oil of Lippia Sidoides; PLGA: Poly D, L-Lactide-Co-Glycolyde; PTFE: Poly...

Determination of sildenafil by various methods and in various media is reviewed in this paper. The methods used consist of ultraviolet (UV) spectroscopy, thin layer chromatography (TLC), and high-performance liquid chromatography (HPLC). These methods were used to determine the amount of sildenafil in pharmaceutical preparations, soft drinks, and biological liquids. HPLC was used for evaluation...

One of the most prevalent over-the-counter cold and cough medications is chlorpheniramine maleate (CPM)–ibuprofen (IBF) combination. A reversed-phase high-performance liquid chromatography (RP-HPLC) method was effectively optimized developed for simultaneous detection ibuprofen in a pharmaceutical formulation. The mobile phase RP-HPLC an isocratic combination acetonitrile 0.01 M acetate buffer ...

Simple, accurate and precise ultraviolet spectrophotometric and reversed-phase high-performance liquid chromatographic (RP-HPLC) methods for simultaneous estimation of amlodipine besylate (AMLO) and olmesartan medoxomil (OLME) in combined tablet dosage form have been developed and validated. Beer’s law was obeyed in the concentration range of 5-70 and 5-60 μg/mL in methanol at 238 nm and 257 nm...

High-performance liquid chromatography (HPLC) with UV, circular dichroism (CD) and intrinsic fluorescence detection was applied to monitor conformational properties of recombinant human interferon alpha2b when performing size exclusion chromatography (SEC) and reversed-phase HPLC (RP-HPLC). In this way native conditions during SEC and structural changes of the protein during RP-HPLC were demons...

Hyphenating High Temperature High Performance Liquid Chromatography (HT-HPLC) with High Temperature Size Exclusion Chromatography (HT-SEC) (High Temperature Two Dimensional Liquid Chromatography (HT-HPLC x HT-SEC or HT 2D-LC)) leads to an isocratic elution in the second dimension, which in turn enables to use IR detector (quantitative detection) for monitoring the eluting polymers. Experimental...

Three different HPLC detection systems were compared for the determination of tocopherols and tocotrienols in olive oil: fluorescence and diode array connected in series, ultraviolet, and evaporative light scattering. The best results were obtained with the fluorescence detector, which was successfully applied in the quantification of tocopherols and tocotrienols in 18 samples of Portuguese oli...

A comprehensive study was conducted to evaluate the persistence of thiram in water and soil under controlled conditions and on two plants, namely tomato and radish, in field conditions. In order to follow the decay of the pesticide, an HPLC procedure was developed employing an octadecyl endcapped RP-C18 column using a mixture of acetonitrile and water as the mobile phase and an ultraviolet dete...