The filamentous fungus Stachybotrys chartarum is known for its toxic metabolites and has been associated with serious health problems, including mycotoxicosis, among occupants of contaminated buildings. Here, we present results from a case study, where an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for known and tentatively identified...

The present study was designed to systematically investigate the chemical profile differences between crude Anemarrhenae rhizoma (CAR) and salt-processed Anemarrhenae rhizoma (SAR). Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS), coupled with multivariate statistical analysis was used for the discrimination of chemical profiles and the i...

A sensitive and reliable method was developed and validated for the determination of unsaturated bile alcohols in sea lamprey tissues using liquid-liquid extraction and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The liver, kidney, and intestine samples were extracted with acetonitrile and defatted by n-hexane. Gradient UHPLC separation was performed usi...

Ultra-high pressure liquid chromatography coupled to high resolution mass spectrometry (UHPLC-MS/MS) analysis of the organic extract obtained from the Mediterranean zoanthid Parazoanthus axinellae yielded to the identification of five new parazoanthines F-J. The structures were fully determined by comparison of fragmentation patterns with those of previously isolated parazoathines and MS/MS spe...

In this series of two papers, 192 doping agents belonging to the classes stimulants, narcotics, cannabinoids, diuretics, β2-agonists, β-blockers, anabolic agents, and hormone metabolic modulators were investigated, with aim assess benefits limitations ion mobility spectrometry (IMS) in combination ultra-high performance liquid chromatography (UHPLC) high resolution mass (HRMS) anti-doping analy...

The aim of this work was to expand the applicability range UHPSFC series synthetic and commercialized peptides. Initially, a screening different column chemistries available for analysis performed, in combination with additives either basic or acidic nature. an additive (13 mM TFA) stationary phase (Torus DEA 2-PIC) found be best six peptides possessing acidic, neutral isoelectric points. Secon...

Ochratoxin A (OTA), one of the most toxic mycotoxins, can contaminate a wide range of food and feedstuff. To date, the data on its conjugates via glucuronidation request clarification and consolidation. In the present study, the combined approaches of ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), UHPLC-Orbitrap-high resolution mass spectrometry (HRMS) and ...

The Agilent 1260 Infi nity Hybrid SFC/UHPLC System offers a fast, reliable, green, robust, and cost-effective solution by coupling the advantages of HPLC and the benefi ts of supercritical fl uid chromatography (SFC). This Application Note describes the conversion of the United States Pharmacopeia (USP) normal-phase HPLC method for cholecalciferol assay to an SFC method using serially coupled c...

An UHPLC-PDA-ESI/HRMS(n) profiling method was used to identify the glucosinolates and flavonoids of Rorippa indica (Cruciferae), a wild vegetable and Chinese herb used to treat cough, diarrhea, and rheumatoid arthritis. Thirty-three glucosinolates, more than 40 flavonol glycosides, and 18 other phenolic and common organic compounds were identified. The glucosinolates and polyphenols were separa...