1. Fragments of breast muscle from a 12 day old chick embryo have been kept alive in single flasks for an entire year without being transferred. The nutrient materials were supplied by frequent applications of adult fowl serum diluted with Tyrode solution. 2. When fragments of fixed tissues are cultivated in serum, cell multiplication and cell death are both reduced to an extremely low level. 3...

The values of membrane action potentials and maximum depolarization rates of single muscle fibers in normal Tyrode solution and in low sodium solutions containing as little as 20 per cent of the sodium chloride were measured with intracellular microelectrodes. Under these conditions the membrane potential remains unchanged up to 36 per cent of [Na(+)](out) concentration, whereas the overshoot o...

Experiments were carried out on the isolated phrenic nerve diaphragm preparation of the rat and isolated sartorius nerve muscle preparations of the frog (Rana tigrina), for the comparison of the neuro-muscular blocking activity. The preparations were set up in 100 ml organ bath containing Tyrode solution at 37°C for rat diaphragm and at room temperature for the frog muscle preparation. The solu...

We compared two methods of zona pellucida drilling. 213 embryos were biopsied with acid Tyrode. Each biopsy took 3 minutes and the entire procedure ~29 minutes. 5% of blastomeres lysed, 49% of embryos became blastocyst and 36% of patients became pregnant. 229 embryos were biopsied with laser. Each biopsy took 30 seconds and the entire procedure ~7 minutes. 2.5% of blastomeres lysed, 50.6% of em...

Chick embryo heart cells were used in coverslip cultures. The medium consisted of: Chick embryo extract 15% Phenol red solution (.04%) 7% Tyrode solution 28% Chicken plasma 50% Cultures were incubated for 72 hours at 37°C. The amount of inhibition of growth was determined from comparisons of the areas of growth and of the mitotic indexes. Many of the compounds were synthesized in this Laborato...

This study was aimed at evaluating the effect of Dihydroartemisinin (DHA)on contractility to drugs in ileum isolated smooth muscle preparations guinea pigs using Vitro experimental procedures / standard protocols an organ bath set up. dihydroartemisinin (1.0 x 10 - 8 1.0 – 5mg/mL) when applied alone and separately excited marked variable effects on pig . In some preparation it showed no respons...

The growth-inhibiting action of serum has been shown to be due largely to the lipoids. Serum from which the lipoids have been removed is much less inhibiting to the growth of fibroblasts in vitro than is the original serum, and only slightly more inhibiting than Tyrode solution. The lipoids extracted from the serum are toxic and more inhibiting to the growth of fibroblasts than the original ser...

Human spermatozoal motility, viability and lipid peroxidation (LPO) have been assessed in Ringer-Tyrode supplemented with different concentrations of ascorbic acid (AA) ranging from 50 to 4000 microM. Ascorbic acid in concentrations below 1000 microM protects spermatozoa from free radical damage as evidenced from improvement in their motility and viability. Concomitantly, there is also witnesse...

Onion (Allium cepa) bulb from Liliaceae has antioxidant, spasmolytic and antihypertensive activities. The aim of present study was to investigate the antispasmodic effect of onion peel on rat ileum contractility. Onion peel powder was extracted by maceration in 70% alcohol for 72 h. A terminal portion of ileum from male Wistar rat was dissected and its contractions were recorded isotonically in...