A total of 706 yeast isolates were evaluated in parallel by the Candida albicans Screen (CAS; Carr-Scarborough Microbiologicals, Inc., Stone Mountain, Ga.) test and the germ tube (GT) test in comparison with the API 20C Yeast Identification System. The CAS and GT tests correctly identified 419 of the 422 isolates of C. albicans (99.3%). Two of the false-negative reactions occurring with the CAS...

Carbon and nitrogen assimilation abilities of yeast isolates are among the first properties tested (2-4) in identifying and classifying yeasts. The recommended tests are conventionally done in liquid medium or by an auxanographic method whereby a solid medium containing all the nutrients and growth factors required by a yeast, except a carbon source, is seeded with the test yeast and then diffe...

canker symptoms similar to those produced by pseudomonas syringae pv. syringae were observed on peach (prunus persica) and nectarine (prunus persicae var. nusipersica) trees in several fruit tree growing areas in khorasan in 2011-2012.symptomatic stem tissues were thoroughly washed under running tap water, surface disinfestated with sodium hypochlorite (2 min. in 0.5 %, ai, hypochlorite), washe...

Becton Dickinson Phoenix Yeast ID Panel was compared to the Remel RapID Yeast Plus System using 150 recent clinical yeast isolates and the API 20C AUX system to resolve discrepant results. The concordance rate between the Yeast ID Panel and the RapID Yeast Plus System (without arbitration) was 93.3% with 97.3% (146/150) and 95.3% (143/150) of the isolates correctly identified by the Becton Dick...

Candida tropicalis is considered as the leading pathogen in nosocomial fungemia and hepatosplenic fungal infections in patients with cancer, particularly in leukemia. The yeast-filament transition is required for virulent infection by Candida. Several studies have explored the genome-wide transcription profile of Candida, however, no report on the transcriptional profile of C. tropicalis under ...

Global-scale data on the Oligocene to Recent planktic foraminifers and coccoliths from the tropical Pacific and Atlantic Oceans are employed for quantitative testing of alternative models (Red Queen and Stationary Hypotheses) of the relationship between speciation rates, extinction rates, taxonomic diversity, abiotic events, and history of the paleosystem. The results demonstrate that although ...

Comprehensive analysis of protein-protein interactions is a challenging endeavor of functional proteomics and has been best explored in the budding yeast. The yeast protein interactome analysis was achieved first by using the yeast two-hybrid system in a proteome-wide scale and next by large-scale mass spectrometric analysis of affinity-purified protein complexes. While these interaction data h...

Rapid identification of yeast isolates from clinical samples is particularly important given their innately variable antifungal susceptibility profiles. We present here an analysis of the utility of PCR amplification and sequence analysis of the hypervariable D1/D2 region of the 26S rRNA gene for the identification of yeast species submitted to the United Kingdom Mycology Reference Laboratory o...

Interactions between proteins are central to most biological processes; consequently, understanding the latter requires identification of all possible protein interactions within a cell. To extend the range of existing assays for the detection of protein interactions, we present a novel genetic screening assay, the cytosolic yeast two-hybrid system (cytoY2H), which is based on the split-ubiquit...