Proteins secreted by Mycobacterium tuberculosis play an essential role in the pathogenesis of tuberculosis. The culture filtrates of M. tuberculosis H37Rv made by Sadamu Nagai (Japan), are considerably enriched for secreted proteins compared to other culture filtrates. Complementary approaches were used to identify the secreted proteins in these culture filtrates: (i) 2-DE combined with MALDI-T...

The Tuberculosis is the classical human mycobacterial disease, caused by Mycobacterium Tuberculosis. Mycobacterium tuberculosis is a facultative intracellular pathogen that has evolved the ability to survive and multiply within human macrophages. These bacteria comprise of significant proteins, which were involve in the pathogenesis and regulation of cell activity. Thus there arises the need to...

The Tuberculosis is the classical human mycobacterial disease, caused by Mycobacterium Tuberculosis. Mycobacterium tuberculosis is a facultative intracellular pathogen that has evolved the ability to survive and multiply within human macrophages. These bacteria comprise of significant proteins, which involve in the pathogenesis and regulation of cell activity. Thus there arises the need to unde...

Tuberculosis is an universal health problem worldwide. In Iraq the problem is aggravated by drug resistance. In Silico studies usually pave the way for more investigations of the real problem .On the other hand Mycobacterium tuberculosis does not lend itself for deep wet lab studies, therefore, In Silico studies must precede many aspects of experimental work. In Silico studies were carried out ...

Two lines of transgenic carrot plants producing Mycobacterium tuberculosis proteins (ESAT6 and CFP10) have been constructed. The target proteins are present in carrot storage roots at a level not less than 0.056% of the total storage protein (TSP) for ESAT6 and 0.002% of TSP for CFP10. As has been shown, oral immunization of mice induces both the cell-mediated and humoral immunities. These data...

background pe5 is a member of the pe protein family whose precise function is yet to be understood. there are about 100 members of the pe family proteins in mycobacterium tuberculosis. these glycine- and alanine-rich proteins consist of proline-glutamate motifs at their n-termini and may be implicated in pathogenesis of the bacilli. objectives we aimed to clone and over-express the rv0285 codin...

The previous studies revealed that Equisetum arvense contained alkaloids, carbohydrate, proteins and amino acids, phytosterols, saponins, sterols, ascorbic acid, silicic phenol, tannin, flavonoids, triterpenoids, volatile oils many other biological active constituents. E. has been used as a folklore medicine for treatment of various conditions such tuberculosis, catarrh in the kidney bladder re...

Exported proteins of bacterial pathogens function both in essential physiological processes and in virulence. Past efforts to identify exported proteins were limited by the use of bacteria growing under laboratory (in vitro) conditions. Thus, exported proteins that are exported only or preferentially in the context of infection may be overlooked. To solve this problem, we developed a genome-wid...