BACKGROUND The importance of wildlife as reservoirs of African trypanosomes pathogenic to man and livestock is well recognised. While new species of trypanosomes and their variants have been identified in tsetse populations, our knowledge of trypanosome species that are circulating in wildlife populations and their genetic diversity is limited. METHODOLOGY/PRINCIPAL FINDINGS molecular phyloge...

Trypanosoma cruzi, a hemoflagellate protozoan (family Trypanosomatidae), is the ethiological agent of Chagas disease, which is affecting 16-18 million people, with more than 100 million exposed to the risk of infection. Higher plants are a potential source of new drugs to improve the treatment of Chagase disease. Until recently, Rutacea, Meliaceae, Simaroubaceae and Burceraceae families have be...

background: the study evaluated qualitative pcr, primers 121-122 as a tool to follow up evolution parasite load of trypanosoma cruzi . methods: the study was conducted at the state university of maringa, in 2015. step 1, dilutions 1/10 were performed from t. cruzi -y strain to obtain preparations of 50,000-0.05 parasites/ml from which dna were extracted, quantified, and amplified. step 2, the e...

trypanosomes infect the blood of the vertebrate host, causing fever, weakness, and lethargy, which lead to weight loss and anemia; in some animals the disease is fatal unless treated and causes serious economic losses. considering the importance of what mentioned present study was conducted to detection of trypanosoma in healthy equids. 116 blood samples collected from lorestan, hamedan, chahar...

Background: Based on the recently sequenced gene coding for the Trypanosoma evansi (T. evansi) RoTat 1.2 Variable Surface Glycoprotein (VSG), a primer pair was designed targeting the DNA region lacking homology to other known VSG genes. A total of 39 different trypanosome stocks were tested using the RoTat 1.2 based Polymerase Chain Reaction (PCR). Results: This PCR yielded a 205 bp product in ...

Accepted: 29/09/2015 Abstract Trypanosoma evansi and T. theileri are the two species of trypanosomes recorded from Karnataka state. The former one is the common species of livestock, dogs and zoo animals, but the latter was rarely reported from bovines. Only low infection rate of clinical cases of T. evansi in bovines was observed based on routine staining of blood smears. The efficacy of wet s...

A study combining DNA sequences of the mitochondrial 16S rRNA gene, advertisement calls and morphology of some southern African river frogs confirms Amietia vandijki (Visser & Channing, 1997) as a good species. The form presently referred to as Amietia angolensis in southern Africa is shown to comprise two species: Amietia angolensis (Bocage, 1866) known from Angola, and Amietia quecketti (Boul...

BACKGROUND The aim of this study was to obtain new data which would be valuable to develop programs for future planning of mosquito controls in in western Iran. METHODS Larvae and adult collections were carried out from different habitats using standard dipping and animal baited trap methods during May, June and July 2012 in two provinces (Kurdistan and Kermanshah) in the west of Iran. Charac...

The anti-Trypanosoma cruzi activity of extracts from 13 Argentinean Asteraceae species was determined. Dichloromethane and methanol extracts of Acmella bellidioides, Aspilia silphioides, Viguiera tuberosa, Calyptocarpus biaristatus, Hyalis argentea, Helenium radiatum, Gaillardia megapotamica, Verbesina subcordata, Gymnocoronis spilanthoides, Viguiera anchusaefolia, Thelesperma megapotamicum, Ze...