Ninety per cent of total rat liver hydrolytic activity (1.4 unitslg of fresh tissue) on diadenosine or diguanosine 5’,5”’-P’,P4-tetraphosphate (Ap4A and Gp4G) present in isotonic homogenates sedimented at 37,000 x g. Supernatant activity corresponded to the earlier described, cytosolic and specific, bis(5’-guanosyl) tetraphosphatase or dinucleoside tetraphosphatase (EC 3.6.1.17; Lobaton, C. D.,...

Aims: T1 /(podoplanin) is abundantly expressed in the alveolar epithelial type I cells (ATI) of rodent and human lungs. Caveolin-1 is a classical primary structural protein of plasmalemal invaginations, so-called caveolae, which represent specialized lipid rafts, and which are particularly abundant in ATI cells. The biological functions of T1 in the alveolar epithelium are unknown. Here we repo...

Iron, cobalt and copper are metals, which appear together in many real samples, both natural and artificial. Recently a classical univariate micellar colorimetric method has been developed for determination of these metal ions. The organized molecular assemblies such as micelles are used in spectroscopic measurements due to their possible effects on the systems of interest. The ability of mi...

Purified Photosystem I reaction center preparation catalyzed a plastocyanin-dependent cytochrome f photooxidation. Euglena cytochrome 552 replaced plastocyanin in all photochemical reactions catalyzed by the purified reaction center and was also photooxidized. A reaction center preparation lacking Subunit III was obtained by means of Triton X-100 treatment and DEAEcellulose chromatography or su...

The influence of sex on systolic blood pressure (SBP) and heart rate (HR) responses associated with cardiovascular morbidity, in healthy young adults was determined in ninety healthy young adults (47 females and 43 males) exercised using Bruce protocol. SBP and HR were measured pre- and post-exercise, and during recovery. SBPresponse (peak minus pre-exercise SBP), %SBPresponse [(peak minus pre-...

Recently, the API X100 steel has emerged as an important pipeline material for transportation of crude oil and natural gas. At the same time, the presence of significant amounts of hydrogen sulfide (H2S) in natural gas and crude oil cause pipeline materials to corrode, which affects their integrity. In this study, the effect of H2S concentration on the corrosion behavior of API X100 in 3.5% NaC...

An electroimmunoassay was applied to the quantitation of platelet-associated IgG (PAIgG). Protein solubilized by Triton X100 from washed platelets was electrophoresed at pH 5.0 in agarose gels containing carbamoylated rabbit anti-human IgG (pI approximately equal to 5.0). Because the rabbit antibody is immobilized under these conditions, while PAIgG is freely mobile, rocket precipitates were pr...

The study of alveolar macrophages (AM) from smokers by flow cytometry (FCM) has been limited by strong autofluorescence and the lack of reliable markers to identify macrophages. Crystal violet quenching was reported to be effective in reducing autofluorescence of AM. CD68 is a marker for macrophages in immunohistochemistry, but has been less useful in FCM because of poor surface expression. Thi...