BACKGROUND In this study we evaluated the RespoCheck Mycoplasma triplex real-time PCR for the detection in bronchoalveolar lavage fluid (BALF) of Mycoplasma (M.) dispar, M. bovis and M. bovirhinis, all three associated with bovine respiratory disease (BRD). Primers and probes of the RespoCheck Mycoplasma triplex real-time PCR are based on the V3/V4 region of the 16S rRNA gene of the three Mycop...

MOTIVATION Current methods for identification of potential triplex-forming sequences in genomes and similar sequence sets rely primarily on detecting homopurine and homopyrimidine tracts. Procedures capable of detecting sequences supporting imperfect, but structurally feasible intramolecular triplex structures are needed for better sequence analysis. RESULTS We modified an algorithm for detec...

We classify all connected, simple, 3-regular graphs with girth at least 5 that are Ricci-flat. use the definition of Ricci curvature on given in Lin-Lu-Yau, Tohoku Math., 2011, which is a variation Ollivier, J. Funct. Anal., 2009. A graph Ricci-flat, if it has vanishing edges. show, only Ricci-flat cubic Petersen graph, Triplex and dodecahedral graph. This will correct classification Comm. Anal...

The interaction of ethidium, a DNA intercalator, with the poly(dA).poly(dT) duplex and the poly (dA).2poly(dT) triplex has been investigated by a variety of spectrophotometric and hydrodynamic techniques. The fluorescence of ethidium is increased when either the duplex or triplex form is present. Binding constants, determined from absorbance measurements, indicate that binding to the triple hel...

MOTIVATION Commercial ultrasound machines in the past did not provide the ultrasound researchers access to raw ultrasound data. Lack of this ability has impeded evaluation and clinical testing of novel ultrasound algorithms and applications. OBJECTIVES Recently, we developed a flexible ultrasound back-end where all the processing for the conventional ultrasound modes, such as B, M, color flow...

The control of gene transcription by antigene oligonucleotides rests upon the specific recognition of doublehelical DNA by triplex-forming oligonucleotides. The development of the antigene strategy requires access to the targeted DNA sequence within the chromatin structure of the cell nucleus. In this sudy we have used HIV-1 chronically infected cells containing the HIV provirus as endogenous g...

Peptide nucleic acids (PNAs) have been developed for applications in biotechnology and therapeutics. There is great potential in the development of chemically modified PNAs or other triplex-forming ligands that selectively bind to RNA duplexes, but not single-stranded regions, at near-physiological conditions. Here, we report on a convenient synthesis route to a modified PNA monomer, thio-pseud...

Discovering knowledge from textual sources and subsequently expanding the coverage of knowledge bases like DBPedia or Freebase currently requires either extensive manual work or carefully designed information extractors. Information extractors capture triples from textual sentences. Each triple consists of a subject, a predicate/property, and an object. Triples can be mediated via verbs, nouns,...

phylogenetic analysis has shown that escherichia coli is composed of four main phylogenetic groups (a, b1, b2 and d). characterization of phylogenetic groups is of clinical interest, as group a and b1 generally associated with commensals, whereas most enteropathogenic isolates are assigned to group d, and group b2 is associated with extra-intestinal pathotype. one hundred e. coli strains recove...

In this work, we forge a powerful, easy-to-visualize, flexible, consistent, and disciplined abstract vector framework for particle and astro physics that is compliant with the holographic principle. We demonstrate that the structural properties of the complex number and the sphere enable us to introduce and define the triplex number—an influential information structure that is similar to the 3D...