Trichomonas vaginalis infections are usually asymptomatic or can result in nonspecific clinical symptoms, which makes laboratory-based detection of this protozoan parasite essential for diagnosis and treatment. We report the development of a battery of highly sensitive and specific PCR assays for detection of T. vaginalis in urine, a noninvasive specimen, and development of a protocol for diffe...

The treatment of trichomonal infestations of the genito-urinary tracts of both men and women has been greatly improved since the introduction of metronidazole in 1959. Cure rates in women of over 80 per cent, have been reported by several observers (Rodin, King, Nicol, and Barrow, 1960; Nicol, Barrow, and Redmond, 1960; Watt and Jennison, 1962). Even higher cure rates have been claimed in cases...

Seventy-three men with urogenital trichomonal infection were treated with a single daily dose of 1 g tinidazole or 1.5 g metronidazole. Both treatments gave satisfactory results. Wives who were also infected with Trichomonas vaginalis, were less likely to have a recurrence of the infection after their husbands had been given either drug.

A rapid and specific dot-enzyme immunoassay (DIBA) using monoclonal antibody was introduced for detection of Trichomonas vaginalis antigen in vaginal and urethral materials. The results of DIBA were compared with culture findings of the parasite in 245 female patients. Taking culture as the reference method, DIBA had a sensitivity of 92% and a specificity of 93%. The predictive value of a posit...

Human genital trichomoniasis has. received a good deal of Attention in recent years, but there are still many aspects of this disease which need fuller investigation. This paper, based on work carried out at the London Hospital during the past 2i years, is offered as an aid in elucidating certain of the more important of these problems-namely, the incidence, mechanism of infection, cause of rel...

Broth culture of Trichomonas vaginalis is considered the "gold standard" for the diagnosis of trichomoniasis. Two studies were carried out to evaluate modified Columbia agar (MCA) for the isolation of T. vaginalis from clinical samples. Study I compared isolation on MCA to that on liquid medium with 889 vaginal samples. Out of 63 samples positive for T. vaginalis (7.1% of total), MCA identified...

Although many investigations into Trichomonas vaginalis Donne 1875 and vaginal trichomoniasis have been made since the organism was recognized again as a pathological entity by Hohne in 1916, problems on its pathogenicity, epidemiology and therapeutics are still left in confusion. One of the main reasons of this confusion appears that the bacteria free cultivation of this organism has not been ...

Trichomonas vaginalis was first described by Donne (1836), who observed the organism in purulent secretions in both women and men, and stated that it was not present in normal vaginal secretions. T. vaginalis was successfully cultured in vitro by Lynch (1915), and there have since been a number of publications containing descriptions of the morphological, epidemiological, and pathological aspec...