Protective antigen (PA) from anthrax toxin assembles into a homoheptamer on cell surfaces and forms complexes with the enzymatic components: lethal factor (LF) and edema factor (EF). Endocytic vesicles containing these complexes are acidified, causing the heptamer to transform into a transmembrane pore that chaperones the passage of unfolded LF and EF into the cytosol. We show in planar lipid b...

Borg, A. 2015. Mechanisms and Inhibition of EF-G-dependent Translocation and Recycling of the Bacterial Ribosome. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 1268. 60 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9289-2. The GTPase elongation factor G (EF-G) is an important player in the complex process of protein synthesis ...

In order to define the influence of skeletal protein organization on transmembrane phospholipid movement in erythrocyte membranes, we measured the translocation rate of lysophosphatidylcholine in pathologic red cells. A simple method based on the differential extraction of lysophosphatidylcholine from the red cell membrane by saline and albumin solutions was used to quantitate the translocation...

A simple method based on the differential extraction of lysophosphatidylcholne (LPC) by saline and albumin solutions has been developed to study the factors that influence lipid translocation across intact human erythrocyte membrane. With this assay, the rate of LPC translocation across the bilayer at 37 "C was found to be 1,87%/h (0.0187 h-'). Identical translocation rates were derived for nor...

In order to investigate the effect of Cd and Zn on uptake, concentration and the translocation factor of the Cd and Zn in the rice plant, a factorial experiment was conducted with four factors including two rice cultivars of Vandana and Hashemi, two waterlogged and non-waterlogged conditions and three levels of Zn and Cd (0, 5 and 10 mg kg-1 soil). The experiment was carried out in a randomized...

Signal transmission progresses via a series of transient protein-protein interactions and protein movements, which require diffusion within a cell packed with different molecules. Yeast Hog1, the effector protein kinase of the High Osmolarity Glycerol pathway, translocates transiently from the cytosol to the nucleus during adaptation to high external osmolarity. We followed the dynamics of osmo...

BACKGROUND Calreticulin (CRT) is major Ca 2+ -binding chaperone mainly resident in the endoplasmic reticulum (ER) lumen. Recently, it has been shown that non-ER CRT regulates a wide array of cellular responses. We previously found that CRT was up-regulated during hypoxia/reoxygenation (H/R) and this study was aimed to investigate whether CRT nuclear translocation aggravates ER stress (ERS)-asso...