1. Human transferrin was labelled with Fe(III) in nitrilo triacetate buffer at one of its two metal binding sites by variation of the pH. The A-site was preferentially labelled at pH 7.2, the B-site at pH 9.2. 2. The monoferric transferrin preparations were subjected to preparative isoelectric focussing, isolated from the granulated gels, and checked for homogeneity and purity on an analytical ...

The transferrins are a group of iron-binding glucoproteins migrating in the 3-globulin region on starch gel electrophoresis (Smithies and Hiller, i959). Commonly, there is a single iron-binding band called transferrin C, but faster (B variants) and slower (D variants) migrating bands are occasionally present. They are termed in order of electrophoretic mobility towards the anode Bo, Bo-i, B1, B...

HREE loci controlling the synthesis of serum proteins in the pig have been Tidentified to date. KRISTJANSSON ( 1960a, b) has identified two alleles controlling the synthesis of transferrins in the pig and has demonstrated (1961) three alleles involved in the synthesis of pig haptoglobins. ASHTON (1960) has described three Yhread protein” phenotypes and on the basis of preliminary mating data ha...

Recombinant human transferrin as well as N- and C-terminal half-transferrins, produced in Escherichia coli, are deposited in inclusion bodies by the bacteria. The isolation and purification of the recombinant proteins from these inclusion bodies are described here. The amino acid compositions and N-terminal sequences of the proteins were determined, and found to be in agreement with the known p...

Fetal fluid was studied in mice of the Q, C57BL/McL, C3H/Bi/McL and JU/Fa strains. The amount increases to day 16 of gestation, and then decreases. Amniotic fluid and fetal plasma contain three transferrins, five alpha-fetoproteins and albumin. Protein concentration in both increases during gestation; glucose decreases in amniotic fluid although it rises in fetal plasms; urea levels remain cons...

1. From pooled serum containing genetically homogeneous transferrin C1, transferrin was purified and separated in three fractions (tri-tri, bi-tri- and bi-bi-antennary transferrin C1), using Concanavalin A-Sepharose. 2. Each of these fractions was separated into its sialic acid-dependent subfractions by preparative isoelectric focusing. Sixteen iso-transferrin C1 fractions were obtained, which ...