A study involving the measurement of glutathione S-transferase activities and isoenzyme distributions in human ovarian tumours has been carried out. These tumours have been obtained either at initial debulking surgery, prior to cytotoxic chemotherapy, or at second look laparotomy following chemotherapy. The response rates of these two groups to chemotherapy differ markedly, wtih patients who ha...

An expression vector, pLEF, has been used to produce the intracellular domain (IC) of the human CD95 (Fas/APO-1) apoptosis receptor as a glutathione S-transferase (GST) fusion protein in murine L929 fibroblasts. GST::CD95IC was affinity-purified in a single step using glutathione-Sepharose. Purification of GST::CD95IC from 32P-labelled L929 cells and cleavage with thrombin revealed that CD95IC ...

Bioluminescence resonance energy transfer coupled near-infrared quantum dots using glutathione-s-transferase (GST) tagged luciferase were synthesized as luminescent probes for in vivo imaging.

We found that the typical fluoroquinolone antibiotic enrofloxacin (ENR) and plasticizer di-(2-ethylhexyl) phthalate (DEHP) are often detected simultaneously at high frequencies in environment, but their combined exposure effects on soil animals poorly understood. Here, oxidative stress, DNA damage changes digestibility of earthworm were investigated to reflect toxicological single DEHP ENR eart...

Antisera to each of the three main cytosolic forms of glutathione S-transferase (GST; alpha, mu, and pi) has been used to characterise GST expression by metastatic neuroblastoma in bone marrow trephine biopsies taken from 15 patients at presentation and from five of this group at relapse. There was no correlation between expression of extra-nuclear alpha or mu GST and outcome, and no consistent...

New highly sensitive latent bioluminescent luciferin substrates were designed and synthesized for monitoring mammalian glutathione S-transferase (GST) and Schistosoma japonicum enzyme activities.

The study investigates the role of root glutathione (GSH) content and glutathione S-transferase (GST) activity in the response of hard wheat (Triticum durum Desf. variety Carioca) induced by different doses of phosphorus KH2<span style="font-s...

MZ: Meso-Zeaxanthin; L: Lutein; RZ: RR-Zeaxanthin; AMD: Age-related Macular Degeneration; MD: Macular Degeneration; NAFL: Non-Alcoholic Fatty Liver; NASH: Nonalcoholic Steatohepatitis; DPPH: Diphenyl-2-Picrylhydrazyl; ABTS: 2, 2’-Azino-Bis (3-Ethylbenzothiazoline-6-Sulfonic acid); GST: Glutathione-S-Transferase; UDPGT: Uridine Diphosphate Glucuronyl Transferase; SGOT: Serum glutamic oxaloacetic...

Glutathione transferase (GST) activity and GST isoenzyme composition have been determined for 24 human neoplasms and 6 human tumor cell lines. Substantial activity (40-1010 milliunits/mg protein) was identified in all tumor specimens examined and three of the tumor cell lines. Three tumor cell lines, the human small cell carcinoma line SW2-10S, the Burkitt's lymphoma derived cell line Raji, and...

To determine whether the tissue distribution of glutathione S-transferase (GST) isoenzymes could define the precise nature of renal injury, 13 adult kidneys were studied, using specific antibodies raised against purified isoenzymes. Basic GST stained strongly proximal convoluted tubules and some medullary tubules; acidic GST stained strongly distal convoluted tubules and medullary tubules; neut...