An improved method for quantitating the extent of myosin light chain (P-LC) phosphorylation in small smooth muscle samples is described. Native myosin was isolated from other cellular proteins in a crude supernatant fraction prepared from a few milligrams of bovine tracheal smooth muscle by polyacrylamide gel electrophoresis (PAGE) in the presence of sodium pyrophosphate (PPi). When potassium i...

The hypothesis that up-regulation of bronchial constrictor endothelin receptors in airway smooth muscle cells may contribute to hyperreactivity during airway inflammation was tested in the present study by quantitative endothelin receptor mRNA analysis and functional responses in ring segments of rat trachea and bronchi. Real time reverse transcription polymerase chain reaction was used to quan...

  Objective(s): In regard to the high incidence of asthma and the side-effects of the drugs used, finding novel treatments for this disease is necessary. Our previous studies demonstrated the preventive effect of Nigella sativa extract on ovalbumin-induced asthma. In addition, water-soluble substances of N. sativa extract and methanol fraction of this plant were responsible for the relaxant eff...

background: when we perform surgery in utero, lungs have an appropriate time to decrease magnitude of hypoplasia, with surgery in uterus. methods: six time-dated single-fetus ewes were selected to induce diaphragmatic hernia. fetal lambs were divided proportionally into 2 groups, namely group 1, diaphragmatic hernia and tracheal ligation (tl group), and group 2, diaphragmatic hernia only (nl gr...

  Abstract   Background: Iatrogenic airway injury after endotracheal intubation and tracheotomy   remains a serious clinical problem. In this study we reviewed post-intubation and traumatic tracheal stenosis in 47 patients with a special attention to the cause, hense surgical treatment of the stenosis was performed and the results compared with the literatures.   Methods: Since February 1995 th...

BACKGROUND AND OBJECTIVES Injuries of tracheal mucosa in contact with tracheal tube cuff is a function of cuff pressure and exposure time. This study aimed at analyzing injuries of tracheal mucosa in contact with tracheal tube cuff inflated to reach "seal" pressure or limit 25 cmH2O pressure, below critical 30 cmH2O, to prevent tracheal damage. METHODS This study involved 16 dogs submitted to...

M. synoviae (MS) is an economically important pathogen and the major cause of airsacculitis and infectious synovitis in turkeys. Infection with this pathogen may remain asymptomatic but can render infected birds susceptible to secondary infections. This study was carried out for the molecular detection of MS infection in commercial and backyard turkey flocks in Tehran, Semnan, Isfahan, Qazvin, ...

BACKGROUND Tracheal necrosis is a severe complication of total pharyngolaryngectomy (TPL). The purpose of this retrospective study was to identify risk factors for tracheal necrosis after TPL. METHODS We performed a retrospective chart review of 177 patients who had undergone TPL. The preoperative, operative, and postoperative variables were examined, and possible risk factors for tracheal ne...