Tomato mosaic virus (ToMV), like other eukaryotic positive-strand RNA viruses, replicates its genomic RNA in replication complexes formed on intracellular membranes. Previous studies showed that a host seven-pass transmembrane protein TOM1 is necessary for efficient ToMV multiplication. Here, we show that a small GTP-binding protein ARL8, along with TOM1, is co-purified with a FLAG epitope-tagg...

tomato is one of the most important vegetables in iran and throughout the world with crop loss caused by several viruses. this survey was carried out to detect the viruses causing mosaic symptoms on tomato in yazd city (iran). a total of 451 samples of tomato leaves showing mosaic symptoms were collected from fields and greenhouses during 2012-2013 growing season. collected samples were analyze...

Quantitative Polymerase Chain Reaction (qPCR) is currently the most sensitive technique used for absolute and relative quantification of a target gene transcript, requiring the use of appropriated reference genes for data normalization. To accurately estimate the relative expression of target tomato (Solanum lycopersicum L.) genes responsive to several virus species in reverse transcription qPC...

It has been proposed that cloned plant disease resistance genes could be transferred from resistant to susceptible plant species to control important crop plant diseases. The recently cloned N gene of tobacco confers resistance to the viral pathogen, tobacco mosaic virus. We generated transgenic tomato plants bearing the N gene and demonstrate that N confers a hypersensitive response and effect...

Tobamoviruses encode a silencing suppressor that binds small RNA (sRNA) duplexes in vitro and supposedly in vivo to counteract antiviral silencing. Here, we used sRNA deep-sequencing combined with transcriptome profiling to determine the global impact of tobamovirus infection on Arabidopsis sRNAs and their mRNA targets. We found that infection of Arabidopsis plants with Oilseed rape mosaic toba...

We reported previously that the movement protein (MP) of tomato mosaic tobamovirus is phosphorylated, and we proposed that MP phosphorylation is important for viral pathogenesis. Experimental data indicated that phosphorylation enhances the stability of MP in vivo and enables the protein to assume the correct intracellular location to perform its function. A mutant virus designated 37A238A was ...

A severe disease of tomato was observed in 2010 in a greenhouse in the province of Lecce (Apulia, southern Italy). Plants showed interveinal yellowing and thickening of mature leaves and a bushy appearance of the new growth. Pale-yellow spots, which became sunken and necrotic, were scattered on the fruit surface. Leaf symptoms were reminiscent of those induced by Tomato chlorosis virus (ToCV) o...

Viral attacks on natural populations of herbaceous forage legumes might have adverse effect on seed and herbage production, hence this study was carried out to investigate the type and incidence of viruses occurring on these plants in Nigeria. Protein A-sandwich (PAS) and antigen-coated plate (ACP) enzyme-linked immunosorbent assays (ELISA) were used to detect ten legume viruses from 100 leaf s...

The evolution over the past century of two tobamoviruses infecting populations of the immigrant plant Nicotiana glauca in New South Wales (NSW), Australia, has been studied. This plant species probably entered Australia in the 1870s. Isolates of the viruses were obtained from N. glauca specimens deposited in the NSW Herbarium between 1899 and 1972, and others were obtained from living plants in...