Stem and root tumors and teratomata may be induced in tobacco plants (Nicotiana tabacum L., var. One Sucker) by total debudding. Intact plants or plants only decapitated produced no tumors or teratomata throughout their life time. These findings suggest a possibly important oncological relationship between normal growth and tumorous growth. Prevention of normal regeneration (apical and axillary...

When tobacco (Nicotiana tabacum L.) cultured cells (line BY-2) at the stationary phase were transferred to culture medium that contained cytokinin (benzyladenine) instead of auxin (2,4-dichlorophenoxyacetic acid), proplastids in the BY-2 cells were converted to amyloplasts within 48 h. The data obtained from in vitro transcription assays using isolated plastid-nucleoids (nuclei) strongly sugges...

Ribulose bisphosphate carboxylase/oxygenase was isolated and crystallized from eight plant species. Crystals grew from either of two similar sets of crystallizing conditions: crystals of the enzyme from alfalfa, corn, cotton, potato, spinach, tobacco, and tomato grew from solutions containing phosphate and polyethylene glycol 6000 as a precipitant, and those from potato, tobacco (both Nicotiana...

Zinc finger homeodomain (ZF-HD) transcription factors play significant roles in plant growth and responses to environmental stresses. In this study, 32 ZF-HD genes identified the tobacco (Nicotiana tabacum L.) genome were divided into six groups according phylogenetic analysis with Arabidopsis tomato members. An examination of gene structures conserved motifs revealed relatively exon/intron mot...

Abstract Tobacco ( Nicotiana tabacum L.) is an economic crop and a model organism for studies of plant biology genetics. As allotetraploid generated from interspecific hybridization, tobacco has massive genome (4.5 Gb). Recently, genetic map with 45,081 single nucleotide polymorphism (SNP) markers was constructed using whole-genome sequencing data population including 274 individuals. This prov...

the genetic diversity of 40 tobacco (nicotiana tabacum l.) cultivars was analyzed using 12 issr primers. thecultivars were evaluated for 14 traits using a simple lattice design (7×7) with two replications. of 149 amplified bands, 142 (95.3%) were polymorphic. maximum and minimum bands were generated by primers ubc825 (17 bands and 94.11% polymorphic) and ubc824 (7 bands and 100% polymorphic), r...

Abstract The composition and content of aroma substances in flue-cured tobacco ( Nicotiana tabacum L.) will affect the quality tobacco. To investigate correlation between various K326 before after flue-curing, their impact on diversity, this study employed middle leaves KRK26 as test materials. Samples were collected both flue-curing for untargeted metabolomics analysis. results showed that 584...

Both phenylalanine ammonia lyase and tyrosine ammonia lyase were detected in tobacco (Nicotiana tabacum L. Wisconsin 38) callus. The enzymes were separated from each other by Sephadex G-200 column chromatography. Increased activity of tyrosine ammonia lyase was observed during culture of tobacco callus under shoot-forming conditions, while activity of phenylalanine ammonia lyase increased durin...

background: nicotiana tobacco contains large amounts of alkaloid nicotine. tobacco plant is used for smoking and causes many health problems since it is high in nicotine which is one of the widely-recognized toxic compounds with serious side effects for different body organs. reducing nicotine content of this plant is a way to reduce its health hazards in cigarette smokers. utilizing the new me...