DNA lesions, induced by genotoxic compounds, block the processive replication fork but can be bypassed by specialized translesion synthesis (TLS) DNA polymerases (Pols). TLS safeguards the completion of replication, albeit at the expense of nucleotide substitution mutations. We studied the in vivo role of individual TLS Pols in cellular responses to benzo[a]pyrene diolepoxide (BPDE), a polycycl...

While Chip Multiprocessors (CMP) with Thread-Level Speculation (TLS) have become the subject of intense research, processor designers in industry have reservations about their practical implementation. An often cited complaint is that TLS is too energy-inefficient to compete against conventional superscalars. This paper challenges the commonly-held view that TLS is energy inefficient. We identi...

When a replicative DNA polymerase stalls upon encountering a lesion on the template strand, it is relieved by other low-processivity polymerase(s), which insert nucleotide(s) opposite the lesion, extend by a few nucleotides, and dissociate from the 3'-OH. The replicative polymerase then resumes DNA synthesis. This process, termed translesion replication (TLS) or replicative bypass, may involve ...

This document specifies how to establish secure connection-oriented media transport sessions over the Transport Layer Security (TLS) protocol using the Session Description Protocol (SDP). It defines a new SDP protocol identifier, ’TCP/TLS’. It also defines the syntax and semantics for an SDP ’fingerprint’ attribute that identifies the certificate that will be presented for the TLS session. This...

This document specifies how to establish secure connection-oriented media transport sessions over the Transport Layer Security (TLS) protocol using the Session Description Protocol (SDP). It defines a new SDP protocol identifier, ’TCP/TLS’. It also defines the syntax and semantics for an SDP ’fingerprint’ attribute that identifies the certificate that will be presented for the TLS session. This...

TLS is the most important cryptographic protocol in the Internet. At CRYPTO 2012, Jager et al. presented the first proof of the unmodified TLS with ephemeral Diffie-Hellman key exchange (TLS-DHE) for mutual authentication. Since TLS cannot be proven secure under the classical definition of authenticated key exchange (AKE), they introduce a new security model called authenticated and confidentia...

In this research, the student will do a survey on TLS security, based on a recent paper by Krawczyk, Paterson, and Wee [KPW13]. Security is first shown for the most common mode of TLS, which is the TLS-RSA, under some security assumptions in the random oracle model. The security of other modes of TLS is then derived, and is shown to hold in the standard model.

Translesion synthesis (TLS) employs low fidelity polymerases to replicate past damaged DNA in a potentially error-prone process. Regulatory mechanisms that prevent TLS-associated mutagenesis are unknown; however, our recent studies suggest that the PCNA-binding protein Spartan plays a role in suppression of damage-induced mutagenesis. Here, we show that Spartan negatively regulates error-prone ...

Translesion DNA synthesis (TLS) during S-phase uses specialized TLS DNA polymerases to replicate a DNA lesion, allowing stringent DNA synthesis to resume beyond the offending damage. Human TLS involves the conjugation of ubiquitin to PCNA clamps encircling damaged DNA and the role of this post-translational modification is under scrutiny. A widely-accepted model purports that ubiquitinated PCNA...

Most SSL/TLS-based e-commerce applications employ conventional mechanisms for user authentication. These mechanisms—if decoupled from SSL/TLS session establishment—are vulnerable to manin-the-middle (MITM) attacks. In this paper, we elaborate on the feasibility of MITM attacks, survey countermeasures, introduce the notion of SSL/TLS session-aware user authentication (TLS-SA), and present a proo...