Accumulating evidence suggest a reciprocal interaction between neurosteroids, especially 5alpha-pregnan-3alpha-ol,20-one (3alpha,5alpha-THP, allopregnanolone), and the serotonergic (5-HT) system. Both 5-HT and neurosteroids seem to play an important role in the pathophysiology of major depression. We have previously shown that a 7-d treatment with 3alpha,5alpha-THP drastically increases the spo...

Tamm-Horsfall protein (THP) is a glycoprotein expressed exclusively in thick ascending limbs (TAL) of the kidney. We recently described a novel protective role of THP against acute kidney injury (AKI) via downregulation of inflammation in the outer medulla. Our current study investigates the mechanistic relationships among the status of THP, inflammation, and tubular injury. Using an ischemia-r...

Mammalian urine contains a range of macromolecule proteins that play critical roles in renal stone formation, among which Tamm-Horsfall protein (THP) is by far the most abundant. While THP is a potent inhibitor of crystal aggregation in vitro and its ablation in vivo predisposes one of the two existing mouse models to spontaneous intrarenal calcium crystallization, key controversies remain rega...

Circulating complexes of leukocytes and activated platelets are markers for atherosclerosis, but their interaction with the arterial endothelial lining has not been studied. Therefore, the effect of activated platelets on rolling and adhesion of labeled human THP-1 monocytoid cells to human umbilical vein endothelial cell (HUVEC) monolayers was studied by epifluorescence microscopy in a paralle...

The identification and characterization of cell surface markers present on different subtypes of cells of the immune system can broaden the understanding of immune cell function. The BD LyoplateTM human cell surface marker screening panel is a collection of cell surface marker antibodies designed for screening cells for the expression of 242 human cell surface proteins by flow cytometry or fluo...

BACKGROUND Mutations in Tamm-Horsfall protein (THP), also known as uromodulin, lead to a group of diseases known as the uromodulin storage disorders. Clinically, these diseases present with tubulo-interstitial damage, progressive renal dysfunction, hyperuricaemia, and gout. However, it remains unclear how a mutation in THP, a protein produced in the thick ascending limb, can cause hyperuricaemi...

V-domain Immunoglobulin Suppressor of T cell activation (VISTA) is an inhibitory B7 family immune-checkpoint molecule expressed on myeloid cells. VISTA-KO mice exhibit Psoriasis-like inflammation. Myeloid cells in psoriasis have multiple abnormalities, including aberrancies monocytic myeloid-derived suppressor and adhesiveness. Whether VISTA signaling related to Psoriasis dysfunction unknown. I...

A human monocyte-derived cell line (THP-1) was used as a model to investigate the role of metallothionein (MT) in the cellular physiology of resting and activated monocytes. MT protein levels were reduced in THP-1 cells by transient transfections with an antisense MT expression vector. Antisense mouse MT-1 RNA was constitutively expressed under the control of the H-2Kb (mouse major histocompati...

Macrophages are an important part of the cellular immune system and play a key role during immune responses. Thus, macrophages are interesting targets in basic and clinical research. Primary monocytes or monocyte-derived macrophages do not proliferate on a suitable scale so that their use for functional studies in vitro is limited. Immortal proliferating cell lines, such as the human THP-1 mono...

Los trastornos de la personalidad están escasamente atendidos en práctica clínica real, a pesar que su prevalencia es elevada y causan altas interferencias malestar emocional aquellas personas los padecen. El objetivo este estudio llevar cabo una revisión sistemática sobre el trastorno límite (TLP) histriónico (THP) desde modelos dimensionales, así como las estrategias regulación (RE) empleadas...