A real-time PCR assay for detecting thermophilic Campylobacter spp. directly in chicken feces has been developed. DNA was isolated from fecal material by using magnetic beads followed by PCR with a prealiquoted PCR mixture, which had been stored at -18 degrees C. Campylobacter could be detected in less than 4 h, with a detection limit of 100 to 150 CFU/ml, in a fecal suspension. A bacterial int...

campylobacter is one of the main potentials for causing diarrhea and gastroenteritis in human saityand. one of the main sources of these bacteria is meat. method and material: 100 samples of broiler’s meat which were slaughtered in slaughter halls all over rafsanjan city were collected during the first 5 months of the year 1389. this study was conducted to determine the prevalence and antimicro...

The emergence of antimicrobial resistance in Campylobacter spp. has been a growing public health concern globally. The objectives of this study were to determine the prevalence, antimicrobial susceptibility, and genetic relatedness of Campylobacter spp. recovered by the National Antimicrobial Resistance Monitoring System (NARMS) retail meat program. Retail meat samples (n = 24,566) from 10 U.S....

Thermophilic campylobacters were isolated from three sewage plants in Rio de Janeiro, RJ, Brazil and identified. Laboratory analysis of 390 sewage samples showed the presence of 169 thermophilic strains. The results demonstrated that human and animal pathogenic biotypes could be isolated from activated sludge during the initial processing steps. The aeration tank could be considered a barrier t...

Campylobacter is one of the most important foodborne pathogens that cause bacterial gastroenteritis.This study was conducted to investigate the prevalence and antimicrobial resistance of Campylobacter in conventional and organic retail poultry samples purchased from grocery stores in Tulsa, Oklahoma.One hundred and fifty six chilled retail chicken samples (85 conventional and 71 organic) and 65...

conclusions campylobacter is responsible for some important clinical problems such as enteritis and is also associated with meningitis and hemolytic-uremic syndrome. it is imperative to monitor the prevalence and antibiotic resistance of campylobacter spp. as well as other the zoonotic bacteria. results twelve (10%) campylobacter spp. from 120 stool samples were isolated including c. coli and c...

The occurrence and species distribution of thermophilic Campylobacter was investigated in organic outdoor pigs. An increased exposure of outdoor pigs to C. jejuni from the environment may cause a shift from a normal dominance of C. coli to more C. jejuni, which may imply a concern of reduced food safety. Bacteriological methods for determination of Campylobacter excretion level were combined wi...

Sierra-Arguello Y.M., Morgan R.B., Perdoncini G., Lima L.M., Gomes M.J.P. & Nascimento V.P. 2015. Resistance to β-lactam and tetracycline in Campylobacter spp. isolated from broiler slaughterhouses in southern Brazil. Pesquisa Veterinária Brasileira 35(7):637-642. Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bent...

the objective of this study was to investigate the genotypic characteristics of campylobacter isolates in shiraz, iran. a total of 40 campylobacter isolates including 20 c. jejuni and 20 c. coli were recovered from both cattle and sheep faeces by cultivation methods. the isolates were identified on the basis of polymerase chain reaction (pcr) detection of 16srrna and multiplex pcr to determine ...

BACKGROUND Campylobacter species are gram negative and flagellated bacteria under the genus Campylobacter, family Campylobacteriaceae. These pathogens cause zoonotic infections among human and animal populations. This study was undertaken between December 2006 and May 2007 to determine prevalence, risk factors and genetic diversity of thermophilic Campylobacter isolates from children less than ...