The use of ionic liquids (ILs) as a solvent for thermal lens measurements has been investigated. It was found that ILs provide a better medium for thermal lens measurements than water. Specifically, not only the ILs offer at least 20 times higher sensitivity than water but that the enhancement can be appropriately adjusted by changing either the cation or the anion of the ILs. For example, the ...

Aquaporin 0 (AQP0) formerly known as membrane intrinsic protein (MIP), is expressed exclusively in the lens during terminal differentiation of fiber cells. AQP0 plays an important role not only in the regulation of water content but also in cell-to-cell adhesion of the lens fiber cells. We have investigated the thermal stress-induced structural alterations of detergent (octyl glucoside)-solubil...

PURPOSE To characterize the optical properties of lenses from mice deficient in the gene for lens intrinsic membrane protein-2 (Lim2), which encodes the second most abundant integral protein (Lim2) of lens fiber cell plasma membranes. METHODS Lim2-deficient mice were derived from a library of gene-trap embryo stem cells. Genotyping was performed by polymerase chain reaction (PCR) amplificatio...

We explore the intuitive lensing picture of laser-heated nanoparticles occurring in single particle photothermal (PT) microscopy. The effective focal length of the thermal lens (TL) is derived from a ray-optics treatment and used to transform the probing focused Gaussian beam with ABCD Gaussian matrix optics. The relative PT signal is obtained from the relative beam-waist change far from the TL...

In recent times, nanofluids have been studied by their thermal properties due to their variety of applications that range from photothermal therapy and radiofrequency hyperthermia (which have proven their potential use as coadjutants in these medical treatments for cancer diseases) to next-generation thermo-fluids. In this work, photoacoustic spectroscopy for a specific study of thermal diffusi...

The cyclodestructive effects of cyclocryotherapy and of the neodymium:yttrium aluminum garnet (Nd:YAG) and diode laser transscleral cyclophotocoagulation were investigated in phakic and pseudophakic cadaver eyes using a modified Miyake posterior-view technique and light microscopy. Cyclocryotherapy to -80 degrees C was applied with a 2.5-mm diameter tip, 1 and 2 mm from the limbus. Freezing at ...

This study reports a novel detection method of Thermal Lens Microscopy (TLM), in which the excitation of analytes is manipulated by Optical Near-Field (ONF) generated on nanostructures. Owning to the property of apparent higher energy than incident photons, herein the ONF allows the electronic excitation of molecules even though the energy of incident light is lower than absorption energy of mo...

Protocols for light microscopy of frog lenses that result in good visibility of cell structure from the lens surface to its nucleus are presented. The lenses are fixed in 10% neutral formalin in 0.06 M phosphate buffer and are embedded in Epon 812. Following staining of the thick sections with selected Procion dyes, essentially every cell in the section plane of the lens can be visualized by si...