Colletotrichum trifolii is the fungal pathogen of alfalfa that causes anthracnose disease. For successful plant infection, this fungus must undergo a series of morphological transitions following conidial attachment, including germination and subsequent differentiation, resulting in appressorium formation. Our previous studies with pharmacological effectors of signaling pathways have suggested ...

Sequences of nodD, a gene found only in rhizobia, were amplified from total community DNA isolated from a pasture soil. The polymerase chain reaction (PCR) primers used, Y5 and Y6, match nodD from Rhizobium leguminosarum biovar trifolii, R. leguminosarum biovar viciae and Sinorhizobium meliloti. The PCR product was cloned and yielded 68 clones that were identified by restriction pattern as deri...

Liriomyza trifolii is a highly-invasive leafmining insect that causes significant damage to vegetables and horticultural crops worldwide. Relatively few studies have quantified gene expression in L. trifolii using real-time quantitative PCR (RT-qPCR), which is a reliable and sensitive technique for measuring gene expression. RT-qPCR requires the selection of reference genes to normalize gene ex...

An undescribed Curvularia sp. was isolated from the leaf spot disease of Barbados Lily (Hippeastrum striatum (Lam.) Moore). Phylogenetic analyses of combined ITS, 28S, GPD1 and TEF1 sequence data place nine strains of this species in the trifolii-clade, but they clustered together as an independent lineage with strong support. This species was morphologically compared with related species in th...

First report of 'Candidatus Phytoplasma trifolii' associated with leaf reddening and upright growth in pears (Pyrus communis L.) | Mustafa Usta, Abdullah Güller, Hikmet Murat Sipahioğlu Agricultural Journals

As a result of pharmacognostic and pharmacological studies, diagnostic signs Trifolii pratense grass collected in the phenophase active flowering area southern Ukraine were revealed. We have proposed adoption an appendix to existing monograph State Service on Medicines Drugs Control “Trifolii inflorescences”, with raw material , appropriate microscopic identification leaves stems plant. Accordi...

In the framework of study on rust fungi of Fabaceae and based on the study of herbarium and newly collected specimens, six Uromyces species are recognized on Medicago spp. and Trifolium spp. from Iran. Among these species, U. fallens is recorded as new member for the Iranian rust flora. U. striatus is recorded as main causal agent of rust on lucerne (Medicago sativa) in Iran. Moreover, U. trifo...

Medicago truncatula is attacked by a broad range of insect herbivores including sieve-element feeders, cell content feeders, and chewing insects. In M. truncatula, genetic resistance against insects has to date been identified only against aphids: single dominant resistance genes have been identified conferring resistance against the pea aphid, bluegreen aphid, and spotted alfalfa aphid. Aphids...

Previously, we have shown that the nodE gene is a major determinant of the difference in host range between Rhizobium leguminosarum biovars viciae and trifolii. A new genetic test system for stringent functional analysis of nodE genes was constructed. By testing chimeric nodE genes constructed by the exchange of polymerase chain reaction (PCR)-generated restriction cassettes, we show that a cen...