Background: The aim of this study was to compare two vitrification systems (Cryotop and OPS) on mouse MII oocytes and evaluate its effects on expression of heat shock protein A1A and MnSOD genes. Materials and Methods: Groups of about 15 oocytes without cumulus complexes (granulose, et. al.) were collected and exposed to equilibration and vitrification solutions before loading the oocytes into ...

introduction: to evaluate the quick cryopreservation technique on parameters of motility, morphology, and vitality of spermatozoa from normozoospermic ejaculates. materials and methods: in this prospective study, a total of 37 normal ejaculates were evaluated according to who criteria, then each sample was divided into two aliquets and sample was cryopreserved with quick freezing method. sperm ...

In this study, a survey was conducted to both evaluate the consumers' general attitudes for purchasing and storing the raw chicken and determine the thawing practices used for defrosting frozen chicken at home. About 75% of the consumers indicated purchasing chicken meat at least once a week or more. Furthermore, the majority (82.16%) of those who stored at least a portion of the raw chicken st...

BACKGROUND/AIMS To observe the effects of vitrification on spindle, zona pellucida, embryonic aneuploidy and DNA injury in in vivo-maruted, in vitro-mature and immature human oocytes. METHODS Between January 2009 and February 2015, 223 immature oocytes from 450 infertile patients, and 31 in vivo-mature oocytes from 3 infertile couples were collected. Of the 223 immature oocytes, 113 were used...

Preservation of bacteria at the temperature of dry ice (-78.5 C) is a particularly valuable method for the maintenance of such organisms as Treponema pallidum which otherwise require continuous animal-to-animal passage. Turner (1938), Turner and Fleming (1939), and Turner and Brayton (1939) demonstrated that at the temperature of dry ice T. pallidum and relapsing fever spirochetes retain their ...

Effects of the timing of soil thawing in the spring on Scots pine (Pinus sylvestris L.) were studied under controlled laboratory conditions. Sixteen 6-year-old saplings were lifted from the field, replanted in containers and placed in four treatments in controlled environment (CE) chambers with four replicate saplings per chamber. The saplings were held in the CE chambers during one simulated w...

The aim of this study was to evaluate the effects of rapid cooling prior to freezing on frozen-thawed canine sperm quality. In experiment 1, centrifuged ejaculates from 6 dogs were pooled, split into 4 aliquots and cryopreserved by the Uppsala procedure using different cooling rates (control, cooling speed 18 C/90 min and average cooling rate 0.2 C/min; rapid, cooling speed 18 C/8 min and avera...

AIM To evaluate different thawing temperatures and duration on the post-thaw semen quality of Indian yaks bulls. MATERIALS AND METHODS Semen ejaculates from four different yak bulls were collected using artificial vagina method and extended with tris extender containing 6.4% glycerol at 35°C, cooled gradually from 35°C to 5°C at 1°C/3 min and equilibrated at 4-5°C for 4 h and frozen in French...

BACKGROUND AND OBJECTIVES With traditional techniques the thawing time for fresh-frozen plasma (FFP) is about 20-30 min. A new technology using radio waves, radio wave thawing device (RTD), was applied for thawing FFP. With this technology the thawing time can substantially be reduced. The new technique was compared to an established method using Heated Air Technology (HAT). Variables subjected...