We and others have recently shown that loss of the mitochondrial membrane potential (Deltapsi) precedes apoptosis and chemical-hypoxia-induced necrosis and is prevented by Bcl-2. In this report, we examine the biochemical mechanism used by Bcl-2 to prevent Deltapsi loss, as determined with mitochondria isolated from a cell line overexpressing human Bcl-2 or from livers of Bcl-2 transgenic mice....

Oxidatively modified LDL mimics several aspects of atherogenesis. In this disease, degradation of the matrix proteins' network also occurs. By a new morphological ex vivo approach, not requiring sample processing, we explored the relationship between the degradation of matrix protein and oxidatively modified LDL. Two-photon excitation fluorescence microscopy images of fresh cross-section rings ...

We previously showed that the cell-permeant antioxidant 2(3)- tert-butyl-4-hydroxyanisole (BHA) inhibited germinal vesicle breakdown (GVBD) in oocyte-cumulus complexes (OCC) of the rat. The objective of the present studies was to assess other antioxidants and whether such inhibition was reversible. Spontaneous GVBD in OCC incubated for 2 h was significantly inhibited ( P < 0.005) by nordihydrog...

Recent studies suggest that reactive oxygen species (ROS) are functional messenger molecules in central sensitization, an underlying mechanism of persistent pain. Because spinal cord long-term potentiation (LTP) is the electrophysiological basis of central sensitization, this study investigates the effects of the increased or decreased spinal ROS levels on spinal cord LTP. Spinal cord LTP is in...

Mice deficient in the electron transport chain (ETC) complex IV assembly protein SURF1 have reduced assembly and activity of cytochrome c oxidase that is associated with an upregulation of components of the mitochondrial unfolded protein response (UPR(MT)) and increased mitochondrial number. We hypothesized that the upregulation of proteins associated with the UPR(MT) in response to reduced cyt...

The novel bicyclic pentadentate ligand 5-methyl-1,5,9,24,25-pentaazapentacyclo[7.7.7.5.5]pentacosane11,13,15,18(25),20,22-hexene (L1) has been synthesized. Because of its cross-bridged topology it exhibits a relatively rigid preorganized conformation especially appropriate to complex formation, as shown by the crystal structure of the monoprotonated ligand salt, HL1ClH2O [orthorhombic, P212121,...

Stabilized melanocortin analog peptide ACTH(6–9)PGP (HFRWPGP) possesses a wide range of neuroprotective activities. However, its mechanism action remains poorly understood. In this paper, we present study the proproliferative and cytoprotective activity adrenocorticotropic hormone fragment 6–9 (HFRW) linked with prolyine–glycyl–proline on SH-SY5Y cells in model oxidative stress-related toxicity...

covalent grafting of mcm-41 with 3-chloropropyl trimethoxysilane and subsequent reaction respectively with acacdien and complexation with moo2(acac)2 afforded moo2acacdien@mcm-41. x-ray diffraction and nitrogen sorption analyses revealed the preservation of the textural properties of the support as well as accessibility of the channel system despite sequential reduction in surface area, pore vo...

Nanoscaled VO2 structures were made using a fast microwave-assisted synthesis method in acetophenone as solvent, starting from vanadium pentoxide V2O5. Insights the reaction and purification process elaborated by attenuated total reflection infrared nuclear magnetic resonance spectroscopy, which validated choice of oleyl amine ligand solvent. A straightforward heat treatment inert atmosphere wa...