DNA polymerase ε (pol ε) is believed to be the leading strand replicase in eukaryotes whereas pols λ and β are thought to be mainly involved in re-synthesis steps of DNA repair. DNA elongation by the human pol ε is halted by an abasic site (apurinic/apyrimidinic (AP) site). We have previously reported that human pols λ, β and η can perform translesion synthesis (TLS) of an AP site in the presen...

We study the total least squares (TLS) problem that generalizes least squares regression by allowing measurement errors in both dependent and independent variables. TLS is widely used in applied fields including computer vision, system identification and econometrics. The special case when all dependent and independent variables have the same level of uncorrelated Gaussian noise, known as ordin...

Changes to stems caused by natural forces and timber harvesting constitute an essential input for many forestry-related applications and ecological studies, especially forestry inventories based on the use of permanent sample plots. Conventional field measurement is widely acknowledged as being time-consuming and labor-intensive. More automated and efficient alternatives or supportive methods a...

The purpose of this study was to determine the effect of dynamometer and joint axis misalignment on measured isometric knee-extension moments using inverse dynamics based on the actual joint kinematic information derived from the real-time X-ray video and to compare the errors when the moments were calculated using measurements from external anatomical surface markers or obtained from the isoki...

Lymphangiogenesis associated with tertiary lymphoid structure (TLS) has been reported in numerous studies. However, the kinetics and dynamic changes occurring to the lymphatic vascular network during TLS development have not been studied. Using a viral-induced, resolving model of TLS formation in the salivary glands of adult mice we demonstrate that the expansion of the lymphatic vascular netwo...

Turnip yellow mosaic virus (TYMV) has a genomic plus-strand RNA with a 5' cap followed by overlapping and different reading frames for the movement protein and polyprotein, while the distal coat protein cistron is translated from a subgenomic RNA. The 3'-untranslated region harbors a tRNA-like structure (TLS) to which a valine moiety can be added and it is indispensable for virus viability. Her...

Earthquake force, loading more than structural capacity, cracking, material fatigue and the other unpredicted events were undeniable in the structure life cycle in order that environmental conditions of the structure would be changed and treats health. Damage of structures such as crack, corrosion of the post tension cables from inappropriate grouting of the post tension structures and etc. can...

REEVES, JOHN T., FUHEID S. DAOIJD, AND MICHAEL GENTRY. Growth of the fetal calf and its arterial pressure, blood gases, and hematologic data. J. Appl. Physiol. 32(2): 240-244. 1972.-Fetal growth, arterial pressure, arterial oxygen tensions, and hematologic data in 35 fetal calves were compared with similar data in fetal lambs. The data of the present report suggested that the fetal calf, its or...

Dear Editor, Translesion synthesis (TLS) allows the DNA replication machinery to bypass an unrepaired DNA damage site using special polymerases called TLS polymerases (Fischhaber and Friedberg, 2005). When compared with the replicative polymerases, TLS polymerases have comparatively large active sites to incorporate the base opposite the damaged DNA and low fidelity to ensure progression of syn...

The establishment of dorsoventral polarity in the Drosophila oocyte and future embryo is dependent on the efficient transport of K10 mRNA from nurse cells into the oocyte. To investigate the cis-requirements of K10 mRNA transport, we used a transgenic fly assay to analyze the expression patterns of a series of K10 deletion variants. Such studies identify a 44 nucleotide sequence within the K10 ...