Tenascin, a predominantly mesenchymal extracellular matrix (ECM) glycoprotein has a rather restricted tissue distribution, but until now factors that inhibit its expression have not been identified. Glucocorticoids are known to be beneficial for establishment of myelopoiesis in long-term bone marrow cultures. Tenascin was found to be expressed in the bone marrow, and glucocorticoids were found ...

Tenascin and fibronectin are two major extracellular matrix glycoproteins. They both consist of large disulfide-linked subunits composed of multiple structural domains. More than half of each molecule consists of so-called fibronectin type III repeats, but the other domains differ. Fibronectin is a dimer, whereas tenascin is a hexamer. Often fibronectin and tenascin are colocalized in tissues, ...

The type IIA rat brain sodium channel is composed of three subunits: a large pore-forming alpha subunit and two smaller auxiliary subunits, beta1 and beta2. The beta subunits are single membrane-spanning glycoproteins with one Ig-like motif in their extracellular domains. The Ig motif of the beta2 subunit has close structural similarity to one of the six Ig motifs in the extracellular domain of...

We have investigated the role of glycosaminoglycans in fibronectin matrix assembly and the incorporation of tenascin-C into matrix fibrils. Chinese hamster ovary cell mutants with a total block in heparan and chondroitin sulfate production failed to assemble a fibronectin matrix, and incorporated no tenascin-C. Another mutant with reduced heparan sulfate produced a normal fibronectin matrix but...

A large number of functions have been demonstrated for tenascin-C by antibody perturbation assays and in vitro cell culture experiments. However, these results contrast sharply with the lack of any apparent phenotype in mice with a genetic deletion of tenascin-C. A possible explanation for the lack of phenotype would be expression of some altered but functional tenascin-C in the mutant. We repo...

Purpose: Tenascin is an extracellular matrix glycoprotein which is expressed transiently in wound healing in human skin and animal corneas. In virro and ednbryological studies suggest that tenascin mediates cell adhesion/anti-adhesion, enhancunen~mlubltion of cell proliiation, and promotion of cell migration. At present there are no published data regarding human comeal wound healing, therefore...

The immunohistochemical expression of tenascin was studied in 80 morphologically diagnosed condylomas and cervical intraepithelial neoplasia (CIN) lesions. The results were compared with the human papillomavirus (HPV) DNA subtype, which was determined by HPV dot blot and in situ hybridisation. Tenascin mRNA synthesis was also determined in 10 selected cases by in situ hybridisation. No statisti...

To elucidate which cells are synthesizing tenascin in usual interstitial pneumonia (UIP) we have analyzed thoracoscopic or open lung biopsies from 30 patients with UIP by mRNA in situ hybridization, using (35)S-labeled tenascin RNA probes. The phenotype of the cells expressing tenascin mRNA was confirmed by immunohistochemical stainings of serial sections with antibodies against alpha-smooth mu...

In the early 1980s several labs independently discovered and characterized the glycoprotein that eventually became known as tenascin. Immunohistochemistry revealed tenascin in everyone’s favorite extracellular matrix: around motile cells, at hot spots of proliferation and at sites of branching morphogenesis, near inductive events, in cartilage, tendons and quite prominently in the developing ne...

The localization of the extracellular matrix recognition molecule J1/tenascin was investigated in the crypt-villus unit of the adult mouse ileum by immunoelectron microscopic techniques. In the villus region, J1/tenascin was detected strongly in the extracellular matrix (ECM) between fibroblasts of the lamina propria. It was generally absent in the ECM at the interface between subepithelial fib...