αβ T-cell receptor (TCR) activation plays a crucial role for T-cell function. However, the TCR itself does not possess signaling domains. Instead, the TCR is noncovalently coupled to a conserved multisubunit signaling apparatus, the CD3 complex, that comprises the CD3εγ, CD3εδ, and CD3ζζ dimers. How antigen ligation by the TCR triggers CD3 activation and what structural role the CD3 extracellul...

Background The aim of this study was to assess peripheral measures and central metabolites associated with lipids using magnetic resonance spectroscopy. Results Twelve patients with complex regional pain syndrome (CRPS) and 11 healthy controls participated. Using magnetic resonance spectroscopy, we measured the levels of lipid 13a (Lip13a) and lipid 09 (Lip09) relative to total creatine (tCr) l...

In this paper, an absorber with a resistive film is proposed, which holds stable polarization absorption characteristics at large angles by exploring the principle of structure. The contains three layers: top layer metal patch film, and middle covered patch. thickness structure only 0.5 mm, can be perfectly attached to trihedral corner reflector (TCR) without affecting reflector. By laying on T...

Despite major advances in T cell receptor (TCR) biology and structure, how peptide-MHC complex (pMHC) ligands trigger αβ TCR activation remains unresolved. Two views exist. One model postulates that monomeric TCR-pMHC ligation events are sufficient while a second proposes that TCR-TCR dimerization in cis via Cα domain interaction plus pMHC binding is critical. We scrutinized 22 known TCR/pMHC c...

TCR gene therapy represents a feasible and promising treatment for patients with cancer and virus infections. Currently, this treatment rationale is hampered by diluted surface expression of the TCR transgene and generation of potentially self reactive T-cells, both a direct consequence of mis-pairing with endogenous TCR chains. As we reported previously (Gene Ther 16:1369, 2000; J Immunol 180:...

Background Immunotherapy of cancer is an emerging field with the potential to improve long-term survival. Thus far, adoptive transfer tumor-specific T cells represents effective treatment option for tumors hematological system such as lymphoma, leukemia or myeloma. However, in solid tumors, efficacy low owing immunosuppressive microenvironment, on-target/off-tumor toxicity, limited extravasatio...

It has been of much interest whether there is functional redundancy between the constitutively signaling pre-T␣/TCR␤ (pre-TCR) and ligated TCR␣␤ complexes, which independently operate the two distinct checkpoints during thymocyte development, i.e., the pre-TCR involved in ␤-selection at the CD4 ؊ CD8 ؊ double-negative stage and the TCR␣␤ being crucial for positive/negative selection at the CD4 ...

BACKGROUND Our previous study had amplified antigen-specific full-length TCR α and β genes of clonally expanded T cells in the peripheral blood (PB) of patients with diffuse large B-cell lymphoma (DLBCL). The transfer of T cell receptor (TCR) genes endows T cells with new antigen specificity. Therefore, the aim of this study is to generate diffuse large B cell lymphoma (DLBCL)-specific T cells ...

Recent studies indicate that there may be functional uncoupling of the TCR-CD3 complex and suggest that the TCR-CD3 complex is composed of two parallel signal-transducing units, one made of gamma delta epsilon chains and the other of zeta chains. To elucidate the molecular mechanisms that may explain the functional uncoupling of TCR and CD3, we have analyzed their expression by using flow cytom...

After productive rearrangement of a TCR beta chain gene, CD4-8- double negative (DN) thymocytes express TCR beta polypeptide chains on the cell surface together with pre-T alpha and the CD3 complex forming the pre-TCR. Signals transmitted through the pre-TCR select TCR beta + DN thymocytes for further maturation to the CD4+8+ double positive stage, whereas DN cells that fail to generate a produ...