This Note analyzes the current state of civil law surrounding Telephone Consumer Protection Act (TCPA) and highlights a glaring flaw within practice assigning liability to telephonic solicitors utilizing an automatic telephone dialing system (autodialer): can be subjected even though their actions are not what Congress intended prevent. enacted TCPA in response unique consumer privacy public sa...

In vivo-induced antigen technology is a method to identify proteins expressed by pathogenic bacteria during human infection. Sera from 10 patients convalescing from cholera infection in Bangladesh were pooled, adsorbed against in vitro-grown El Tor Vibrio cholerae O1, and used to probe a genomic expression library in Escherichia coli constructed from El Tor V. cholerae O1 strain N16961. We iden...

The toxR gene of Vibrio cholerae encodes a transmembrane, DNA-binding protein that activates transcription of the cholera toxin operon and a gene (tcpA) for the major subunit of a pilus colonization factor. We constructed site-directed insertion mutations in the toxR gene by a novel method employing the chromosomal integration of a mobilizable suicide plasmid containing a portion of the toxR co...

The toxin-coregulated pilus (TCP) of Vibrio cholerae O1 is required for successful infection of the host. TcpA, the structural subunit of TCP, belongs to the type IV family of pilins, which includes the PilE pilin of Neisseria gonorrhoeae. Recently, single amino acid changes in the N-terminus of PilE were found to abolish autoagglutination in gonococci. As type IV pilins demonstrate some simila...

A localization system is one of the basic requirements for coal mines. Ultra-wideband (UWB), as a technology with broad application prospects, considered to have great potential in absence satellite signals, especially underground mine environment, it can provide rescue assistance. However, state-of-the-art UWB position systems mines cannot efficiently differ line-of-sight from all communicatio...

The aim of this study was to assess the modulatory effect TcpA in expression CEACAM1 adhesin molecule and IL-1, IL-8, TNF-α pro-inflammatory cytokines Coculture model Caco-2/PBMC (peripheral blood mononuclear cell) that can mimic intestinal milieu. gene from Vibrio cholerae ATCC14035 cloned pET-28a transformed into Escherichia coli Bl-21. recombinant TcpA-His6 protein expressed purified using N...

Over the last two years, the computing industry has started various initiatives to increase computer security be means of new hardware. The most notable effort is the Trusted Computing Group (TCG, former TCPA), which unites most important companies to develop a standard for security hardware. While efforts towards more security are generally appreciated, the new technology has raised fears that...