conclusions the quite sensitive in-house taqman real time rt-pcr assay was able to detect and quantify all four main hcv genotypes prevailing around all geographical regions of iran. results the lower limit detection of this in-house hcv real-time rt-pcr was determined as 100 rna copies/ml. inter- and intra-assay coefficient of variation (cv) of this in-house hcv real-time rt-pcr ranged from 0....

Applied Biosystems has designed and manufactured over 40,000 real-time PCR assays for measuring the expression of genes in humans, mice, and rats. TaqMan Gene Expression Assays each consist of amplification primers (forward and reverse) and a fluorescent labeled TaqMan probe, formulated into a single tube. One of the major user concerns regarding any real-time PCR-based assay product (primers a...

Leptospirosis, caused by pathogenic Leptospira, is one of the most important zoonoses in the world. Several molecular techniques have been developed for detection and differentiation between pathogenic and saprophytic Leptospira spp. The aim of this study was to develop a rapid and simple assay for specific detection and differentiation of pathogenic Leptospira spp. by multiplex real-time PCR (...

A new readout approach for the Hamiltonian Path Problem (HPP) in DNA computing based on the real-time polymerase chain reaction (PCR) is investigated. Several types of fluorescent probes and detection mechanisms are currently employed in real-time PCR, including SYBR Green, molecular beacons, and hybridization probes. In this study, real-time amplification performed using the TaqMan probes is a...

The herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2) and varicella-zoster virus (VZV) can cause life-threatening infections of the central nervous system and lead to severe infections in immunocompromised subjects and newborns. In these cases, rapid diagnosis is crucial. We developed three different real-time PCR assays based on TaqMan chemistry for the LightCycler instrument to detect HS...

Blastocystis is a common intestinal parasite of unsettled clinical significance, which is not easily detected by standard parasitological methods. The genus comprises at least 13 subtypes (STs) (which likely represent separate species), 9 of which have been found in humans. Recent data indicate that at least one of the subtypes is associated with intestinal disease. A quantitative TaqMan 5' nuc...